Expression of transforming growth factor β1 and lysophosphatidic acid increasing the activation of integrin αvβ6 in hepatic cancer

doi:10.3877/cma.j.issn.1674-0793.2016.05.005

Abstract

Abstract:

Objective

To investigate whether transforming growth gactor-beta1 (TGF-β1) and lysophospholipids acid (LPA) can induce the overexpression of integrin αvβ6 (Itg αvβ6) in human hepatic cancer Hep-3B.

Methods

Tissue samples were obtained from twenty three patients with hepatocellular carcinoma, and the expression level of Itg αvβ6 was detected by immunohistochemistry. The expression of Itg αvβ6 in Hep-3B cells and normal liver cells HL-7702 was treated with revised transcription PCR (RT-PCR). Hep-3B cells were treated with either TGF-β1, LPA, or TGF-β1+LPA, then Itg αvβ6 expression was examined by real-time PCR and Western blotting.

Results

αvβ6 overexpression was present in the hepatocellular carcinoma, while absent in normal liver tissue. αvβ6 mRNA was detected in Hep-3B cells but not detectable in HL-7702 cells. TGF-β1, LPA and TGF-β1+LPA induced Itg avβ6 overexpression at protein levels and mRNA levels, with a significant difference (P<0.05).

Conclusions

αvβ6 overexpression is present in both the hepatocellular carcinoma and Hep-3B cells. TGF-β1 and LPA induce the Itg αvβ6 overexpression in Hep-3B cells, which will be expected to provide a new potential therapeutic target for hepatocellular carcinoma.

Key words: Carcinoma, hepatocellular, Integrin alpha V beta6, Transforming growth factor beta1, Lysophospholipids

Hui Han, Ruirui Xu, Gengzhen Chen, Chengliang Wu, Kaixin Zheng, Zehong Huang. Expression of transforming growth factor β1 and lysophosphatidic acid increasing the activation of integrin αvβ6 in hepatic cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2016, 10(05): 340-345.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2016.05.005

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