Experimental study of the aloe polysaccharides relieving enteritis by regulating Th17/Treg cell balance

doi:10.3877/cma.j.issn.1674-0793.2019.06.004

Abstract

Abstract:

Objective

To investigate whether aloe polysaccharide from aloe extract can alleviate the enteritis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) via correcting Th17/Treg cell imbalance.

Methods

Thirty SD rats were divided into normal group (enema with phosphate buffered saline), aloe polysaccharide treatment group (enema with TNBS and ethanol and anal injection of aloe polysaccharide) andmodel group (enema with TNBS and ethanol) by complete digital random method, with 10 rats in each group. The bodyweight change and disease activity index (DAI) were recorded. The macroscopic and microscopic damage scoring of colon tissue were examined histopathologically. The myeloperoxidase (MPO) activity, mRNA expression level of cytokine transforming growth factor (TGF-β), interleukin (IL)-10, IL-17A and IL-6, as well as the protein expression of transcription factors Foxp3⁺, RORγt and the signal transducer and activator of transcription (STAT)3, STAT5 were detected in the colon tissue. The expression of IL-1β, tumor necrosis factor alpha (TNF-α) and anti-tumor necrosis factor alpha stimulating gene 6 (TSG-6) in the plasma were detected.

Results

Compared with the model group, DAI, MPO activity, histopathological scores and the protein expression of the plasma cytokines IL-1βand TNF-αin the aloe polysaccharide treatment group were significantly decreased, but the concentration of TSG-6 in the plasma was increased. Aloe polysaccharide inhibited the mRNA expression of pro-inflammatory cytokines IL-17A and IL-6 as well as transcription factor RORγt activation and STAT3 phosphorylation in colon tissue, but promoted the mRNA expression of anti-inflammatory cytokines IL-10, TGF-βas well as transcription factor Foxp3⁺activation and STAT5 phosphorylation.

Conclusion

Aloe polysaccharide can alleviate TNBS-induced experimental enteritis by correcting Th17/Treg cell imbalance.

Key words: Inflammatory bowel disease, Aloe, Polysaccharides, Th17 cells, T-lymphocytes, regulatory

Zhengwei Fu, Lixia Wang, Haiyan Ge. Experimental study of the aloe polysaccharides relieving enteritis by regulating Th17/Treg cell balance[J]. Chinese Archives of General Surgery(Electronic Edition), 2019, 13(06): 435-440.

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References 18

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相对体质量下降(%)	大便隐血实验	大便性状	分值
<1	阴性	正常	0
1~5	隐血阳性	软便	1
>5~10	阳性	大便松散	2
>10~15	阳性	稀糊样便	3
>15	肉眼血便	水样腹泻	4

相对体质量下降(%)	大便隐血实验	大便性状	分值
<1	阴性	正常	0
1~5	隐血阳性	软便	1
>5~10	阳性	大便松散	2
>10~15	阳性	稀糊样便	3
>15	肉眼血便	水样腹泻	4

基因名称	正向引物序列(5’→ 3’)	反向引物序列(5’→ 3’)
IL-17A	AAGCACAGAAAGCATGATCCG	GAGTCCAGGGTGAAGTGGA
IL-10	CCTTCCTCCGTGTGGTTTGA	TTCTTGAGGGCCACTTCGTA
IL-6	GCCAGATAGAGTCGTTGCCC	AGCTAGTTGCCGTGTGTCTG
TGF-β	CCCACATGAGATCAGCCTCC	GGAGTTGGGTGGCAAGAGTT
GAPDH	TCAATGAAGGGGTCGTTGAT	CGTCCCGTAGACAAAATGGT

基因名称	正向引物序列(5’→ 3’)	反向引物序列(5’→ 3’)
IL-17A	AAGCACAGAAAGCATGATCCG	GAGTCCAGGGTGAAGTGGA
IL-10	CCTTCCTCCGTGTGGTTTGA	TTCTTGAGGGCCACTTCGTA
IL-6	GCCAGATAGAGTCGTTGCCC	AGCTAGTTGCCGTGTGTCTG
TGF-β	CCCACATGAGATCAGCCTCC	GGAGTTGGGTGGCAAGAGTT
GAPDH	TCAATGAAGGGGTCGTTGAT	CGTCCCGTAGACAAAATGGT

组别	n	第1天	第2天	第3天	第4天	第5天	第6天	第7天
正常组	10	100.35±0.12	100.58±0.41	100.93±0.62	101.79±1.61	101.88±0.67	102.17±0.55	102.37±0.67
治疗组	10	97.63±1.53	95.91±0.64	94.13±1.23^a	91.88±1.17^a	93.01±0.84^a	94.38±0.97^a	95.29±1.07^a
模型组	10	97.42±1.38	95.83±0.76	92.71±1.55	88.64±2.12	89.14±1.10	89.99±0.97	90.49±1.31
F值	?	14.501	151.281	107.553	131.066	427.723	436.696	256.323
P值	?	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

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