Circular RNA-PCAC1 promotes the metastasis of pancreatic ductal adenocarcinoma

doi:10.3877/cma.j.issn.1674-0793.2020.03.003

Abstract

Abstract:

Objective

To determine the expression of circular RNA (circRNA) and evaluate the value of circRNA for clinicopathologic and prognostic of pancreatic ductal adenocarcinoma.

Methods

The construction of circRNA-PCAC1 was determined by RT-PCR using random primer or oligo-dT primer. The invasion of pancreatic ductal adenocarcinoma cells were measured by transwell assay in vitro. The expression of circRNA-PCAC1 in a cohort of 76 cases of pancreatic ductal adenocarcinoma tissues and paired normal adjacent tissues were detected by RT-PCR. The clinicopathologic information was collected and analyzed for the correlation with circRNA-PCAC1 expression by using Cox regression model and Kaplan-Meier curve analysis.

Results

circRNA-PCAC1 promoted pancreatic ductal adenocarcinoma cells invasion. The expression of circRNA-PCAC1 was up-regulated in pancreatic ductal adenocarcinoma compared with normal adjacent tissues (P<0.01). High expression of circRNA-PCAC1 was associated with poor prognosis in pancreatic ductal adenocarcinoma (P<0.05). circRNA-PCAC1 was an independent poor prognostic predictor related to overall survival (HR=1.733, 95% CI: 1.066-2.991, P=0.030) and disease free survival (HR=1.636, 95% CI: 1.090-2.811, P=0.042) rate of pancreatic patients.

Conclusion

circRNA-PAPC1 is up-regulated in pancreatic ductal adenocarcinoma and promotes the invasion and metastasis of pancreatic ductal adenocarcinoma, supporting that circRNA-PCAC1 may be a potential therapeutic target for pancreatic ductal adenocarcinoma.

Key words: Pancreatic neoplasms, Circular RNA, Neoplasm metastasis, Pancreatic ductal adenocarcinoma

Yuting Li, Yao Kong, Yuming Luo, Hanhao Zheng, Jiang Zhu, Bowen Gao, Le Ai, Rufu Chen, Zhihua Li. Circular RNA-PCAC1 promotes the metastasis of pancreatic ductal adenocarcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(03): 174-179.

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Figures/Tables 7

References 15

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引物名称	序列(5’-3’)
circRNA-PCAC1上游引物	GAGGGTTCTGCTGTCCTGT
circRNA-PCAC1下游引物	GATAGACGCGTGCACCAC
GAPDH上游引物	TGCACCACCAACTGCTTAGC
GAPDH下游引物	GGCATGGACTGTGGTCATGAG
EIF6上游引物	TGCAGATTAGGCGGGTGGAG
EIF6下游引物	GTCGGCCACTGTCTGTCTGA

引物名称	序列(5’-3’)
circRNA-PCAC1上游引物	GAGGGTTCTGCTGTCCTGT
circRNA-PCAC1下游引物	GATAGACGCGTGCACCAC
GAPDH上游引物	TGCACCACCAACTGCTTAGC
GAPDH下游引物	GGCATGGACTGTGGTCATGAG
EIF6上游引物	TGCAGATTAGGCGGGTGGAG
EIF6下游引物	GTCGGCCACTGTCTGTCTGA

临床病理特征		例数	低表达	高表达	χ²值	P值
例数		76	38	38	?	?
性别		?	?	?	0.477	0.490
?	男	41	19	22	?	?
?	女	35	19	16	?	?
年龄(岁)		?	?	?	0.216	0.642
?	≤60	32	17	15	?	?
?	> 60	44	21	23	?	?
分化程度		?	?	?	2.177	0.337
?	低	12	6	6	?	?
?	中	49	22	27	?	?
?	高	15	10	5	?	?
淋巴转移		?	?	?	0.066	0.797
?	无	21	10	11	?	?
?	有	55	28	27	?	?
TNM分期		?	?	?	11.624	0.003^**
?	Ⅰ	12	11	1	?	?
?	Ⅱ	44	21	23	?	?
?	Ⅲ	20	6	14	?	?

临床病理特征		例数	低表达	高表达	χ²值	P值
例数		76	38	38	?	?
性别		?	?	?	0.477	0.490
?	男	41	19	22	?	?
?	女	35	19	16	?	?
年龄(岁)		?	?	?	0.216	0.642
?	≤60	32	17	15	?	?
?	> 60	44	21	23	?	?
分化程度		?	?	?	2.177	0.337
?	低	12	6	6	?	?
?	中	49	22	27	?	?
?	高	15	10	5	?	?
淋巴转移		?	?	?	0.066	0.797
?	无	21	10	11	?	?
?	有	55	28	27	?	?
TNM分期		?	?	?	11.624	0.003^**
?	Ⅰ	12	11	1	?	?
?	Ⅱ	44	21	23	?	?
?	Ⅲ	20	6	14	?	?

影响因素	单因素分析			多因素分析
影响因素	HR	95% CI	P值	HR	95% CI	P值
性别(男 vs女)	1.187	0.698~2.147	0.550	?	?	?
年龄(>60岁 vs ≤60岁)	1.866	0.570~4.097	0.215	?	?	?
分化程度(低+中 vs高)	1.259	0.874~2.149	0.316	?	?	?
淋巴结转移(有 vs无)	1.533	1.012~2.361	0.048	1.314	0.951~2.080	0.171
TNM分期(Ⅱ+Ⅲ vs Ⅰ）	2.143	0.877~5.873	0.116	?	?	?
circRNA-PCAC1表达(高 vs低)	1.916	1.109~3.310	0.020	1.733	1.066~2.991	0.030

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