CXCL1 promoting the metastases of gastric cancer via integrin β1

doi:10.3877/cma.j.issn.1674-0793.2020.04.003

Abstract

Abstract:

Objective

To investigate the effect of CXCL1 on the migration of gastric cancer (GC) cells and its potential mechanism.

Methods

Lentivirus particles expressing CXCL1 were constructed to establish a stable CXCL1 overexpressing SGC-7901 cell line (7901-CXCL1-GFP) and BGC-823 cell line (823-CXCL1-GFP). siRNA was utilized to block the expression of CXCL1 and integrin β1 in SGC-7901 and BGC-823 cells. Western blotting was performed to analyze the expression of CXCL1, integrin β1, FAK,SRC, ERK, MMP-2 and MMP-9. Transwell assays were adopted to evaluate the migration of gastric cancer cells.

Results

The expression of integrin β1 was higher in 7901-CXCL1-GFP and 823-CXCL1-GFPcells than that in 7901-GFP and 823-GFP cells. Integrin β1 expression was down-regulated by blocking CXCL1 with siRNA. Blocking integrin β1 by siRNA could inhibit CXCL1-induced migration of SGC-7901and BGC-823 cells. CXCL1 increased the expression of p-FAK, p-SRC and p-ERK in GC cells, which could be blocked by integrin β1 knockdown with siRNA. CXCL1 induced the expression of MMP-2 and MMP-9 in GC cells through integrin β1 signaling, meanwhile knockdown of integrin β1 inhibited the MMP-2 and MMP-9 expression. GM6001, one MMPs inhibitor, significantly reduced CXCL1-induced migration of 7901-CXCL1-GFP and 823-CXCL1-GFP cells (P<0.05).

Conclusion

Overexpressed CXCL1 promotes migration of GC cells by activating FAK-SRC-ERK pathway and regulating the expression of MMP-2, MMP-9 through integrin β1 signaling.

Key words: Gastric Cancer, Chemokines, CXCL1, Integrin β1

Zhewei Wei, Changhua Zhang, Yulong He. CXCL1 promoting the metastases of gastric cancer via integrin β1[J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(04): 248-252.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2020.04.003

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2020/V14/I04/248

Figures/Tables 5

References 12

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