Investigation of miR-223 in regulating breast cancer cells and osteoclast function via IGF-1R and NFIA

doi:10.3877/cma.j.issn.1674-0793.2020.06.002

Abstract

Abstract:

Objective

To investigate the effect and mechanism of miR-223 in regulating breast cancer cells and osteoclast in bone metastasis microenvironment.

Methods

The role of miR-223 in regulating bone resorption was evaluated by micro-CT and histologic section in miR-223 knockout and C57BL/6 mice. The effect and mechanism of miR-223 in RANKL induced osteoclast formation was investigated. The role and mechanism of miR-223 in regulating breast cancer cell proliferation and apoptosis were investigated in MDA-MB-231 cell line.

Results

The osteoclastic resorption was more severe inmiR-223 knockout mice. In vitro, miR-223 could suppress RANKL activating osteocalst formation by targeting NFIA protein. Besides, overexpression of miR-223 inhibited MDA-MB-231 cell proliferation and promoted its apoptosis by targeting IGF-1R and PI3K/Akt pathway.

Conclusion

miR-223 is a protective factor in bone metastasis microenvironment, which can suppress bone resorption by inhibiting osteoclast formation, and suppress breast cancer cell proliferation, enhance breast cancer cell apoptosis.

Key words: Breast neoplasms, MicroRNAs, Bone metastasi, Osteoclasts

Zhen Shan, Wen Li, Yuanjian Fan, Zefei Lin, Ying Lin, Shenming Wang. Investigation of miR-223 in regulating breast cancer cells and osteoclast function via IGF-1R and NFIA[J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(06): 406-410.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2020.06.002

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2020/V14/I06/406

Figures/Tables 8

References 14

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小鼠类型	n	骨小梁组织体积(mm³)	骨小梁区域体积(mm³)	骨体积分数(BV/TV)	骨矿物密度(mg HA/ccm)
野生型	6	6.17±0.29	0.72±0.04	0.12±0.01	207.46±2.05
miR-223-/-	6	5.11±0.24	0.37±0.07	0.07±0.01	156.12±11.60
t值		-	-	10.04	15.10
P值		-^a	-^a	<0.001	<0.001

小鼠类型	n	骨小梁组织体积(mm³)	骨小梁区域体积(mm³)	骨体积分数(BV/TV)	骨矿物密度(mg HA/ccm)
野生型	6	6.17±0.29	0.72±0.04	0.12±0.01	207.46±2.05
miR-223-/-	6	5.11±0.24	0.37±0.07	0.07±0.01	156.12±11.60
t值		-	-	10.04	15.10
P值		-^a	-^a	<0.001	<0.001

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