Expression and function of circRNA circEIF6 in hepatocellular carcinoma

doi:10.3877/cma.j.issn.1674-0793.2021.04.003

Abstract

Abstract:

Objective

To explore the expression of circRNA circEIF6 in hepatocellular carcinoma (HCC), and its effect on cell proliferation, invasion and migration in HCC.

Methods

Data sets GSE94508 and GSE97332 of circRNAs that differentially expressed between HCC and paracancerous tissues were downloaded from GEO database, and circEIF6, among circRNAs with higher expression in HCC tissues, was selected as the research object. A total of 15 cancer tissues and corresponding para-cancerous tissues were collected from HCC patients who underwent surgical resection in the First Affiliated Hospital of Sun Yat-sen University from January 2015 to December 2018. qPCR analysis was used to test the expression of circEIF6. Then, siRNAs were used to knock down the expression of circEIF6 in Huh7 cell line. CCK8 assay, EdU assay, plate clone formation assay, Transwell assay were conducted to analyze cell proliferation, invasion and migration in HCC.

Results

A total of 81 circRNAs differentially expressed in HCC were screened, and the corresponding maternal genes of 73 circRNAs with up-regulated expression were analyzed by GO. The expression of circEIF6 in liver cancer tissues was significantly higher than that in adjacent liver tissues (P<0.05). When circEIF6 was knocked down by siRNAs, the proliferation, invasion and migration abilities of Huh7 cells decreased significantly (P<0.05).

Conclusion

circEIF6 can promote cell proliferation, invasion and migration in HCC, and the related mechanism needs further study.

Key words: circRNA, Hepatocellular carcinoma, siRNA, Proliferation, Invasion

Yiqin Wang, Shun Li, Xiaofang Zeng, Zhili Ma, Lixia Xu, Ming Kuang. Expression and function of circRNA circEIF6 in hepatocellular carcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(04): 252-257.

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References 19

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