To establish adult rat major pelvic ganglion (MPG) injury model in vitro in order to provide an effective model for basic research of pelvic nerve injury related diseases.

Adult SD rats at 8 weeks were selected and sacrificed to obtain MPG tissue in vitro. The MPG tissue was cultured on RPMI-1640 medium. One week later, hydrogen peroxide (H₂O₂) was added with different concentrations of 0, 100, 200, 300, 400, 500 μmol/L to induce MPG oxidative stress injury. The expression of neural marker was detected by immunohistochemistry. In addition, the level of neurotrophic factors was detected by ELISA, and the growth length of peripheral neurofilament was compared to determine the appropriate concentration of injury induction.

Tissue adhesion and neurofilament growth were observed 3 days after in vitro culture of adult SD rat MPG. The staining of neurofilament protein marker NF-H was positive, which confirmed the regeneration of nerve tissue. With the increase of H₂O₂ concentration, the tissue morphology of MPG revealed different degrees of fragmentation. Meanwhile, the expression of neural marker S100β was down-regulated, and the growth length of neurofilament was inhibited. Accordingly, the expression levels of brain derived neurotrophic factor and nerve growth factor in the cultured medium decreased gradually.

Stable in vitro injury model of MPG in adult SD rats can be established after cultured on RPMI-1640 medium and induced with 200 μmol/L H₂O₂.