To explore the effects of different processing factors on oxaliplatin sensitivity test results of colorectal cancer organoids and provide reference for the individualized precision treatment for tumor patients.

Two cases of colorectal cancer organoids were processed with different cell density, different cell culture time prior to administration and different drug action time. Five doses of oxaliplatin were designed to act on the two cases of colorectal cancer organoids. CCK-8 was used to detect cell proliferation activity. The effects of different processing factors on drug sensitivity test results of colorectal cancer organoids were analyzed according to cell survival rate.

In the selection of cell density, 100 μmol/L oxaliplatin could significantly inhibit the growth of colorectal cancer organoids in both cases when inoculated with 8 000 and 4 000 cells per well, and there was no significant difference between the two groups. When inoculated with 2 000 cells per well, the growth inhibitions of 100 μmol/L oxaliplatin on colorectal cancer organoids in both cases were significantly weakened (compared with 8 000 and 4 000 cells per well, P<0.05), and the results of efficacy evaluation became invalid in vitro. No regular results were obtained on the time of cell culture prior to administration. In the choice of time of action, 100 μmol/L oxaliplatin showed more significant growth inhibition effect on the two colorectal cancer organoids after being treated for 7 days than 3 days (P<0.05).

Different cell density, different cell culture time prior to administration and different drug action time show influence on the drug sensitivity test results of colorectal cancer organoids. Different experimental conditions may lead to opposite efficacy evaluation results.