Preparation of a novel VEGFR-targeting probe second near-infrared window in a mouse model of colorectal cancer

doi:10.3877/cma.j.issn.1674-0793.2023.03.003

Abstract

Abstract:

Objective

To investigate the imaging effect of the second near-infrared window (NIR-Ⅱ, 1 000-1 700 nm), a novel VEGFR-targeting probe ICG-PEG5000-Bev in a mouse model of colorectal cancer.

Methods

ICG-PEG5000-Bev, a novel VEGFR-targeting probe in NIR-Ⅱ region, was prepared to verify its spectral characterization and biosafety. The in vivo imaging effect and application prospect of ICG-PEG5000-Bev was further explored by using the NIR-Ⅱ imaging device in mice model with CT-26-luc colorectal cancer.

Results

ICG-PEG5000-Bev had good biological safety. Spectral experiments showed that it had NIR-Ⅱ imaging ability, and the fluorescence intensity increased linearly with the increase of probe solution concentration within a certain range. The NIR-Ⅱ imaging effect of the probe showed that ICG-PEG5000-Bev could achieve in vivo imaging of mice with colorectal cancer, with lower background signal strength than NIR-Ⅰ .

Conclusion

ICG-PEG5000-Bev can be used to detect colorectal cancer in mice in vivo with good biosafety and strong clinical transformation value.

Key words: Vascular endothelial growth factor receptor, Colorectal neoplasms, Fluorescence imaging, The second near-infrared window

Bo Wang, Zirui Bai, Jian Li. Preparation of a novel VEGFR-targeting probe second near-infrared window in a mouse model of colorectal cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(03): 173-177.

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Figures/Tables 8

References 15

[1]	Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249.
[2]	Siegel RL, Miller KD, Goding Sauer A, et al. Colorectal cancer statistics, 2020[J]. CA Cancer J Clin, 2020, 70(3): 145-164.
[3]	中华人民共和国国家卫生健康委员会. 中国结直肠癌诊疗规范(2020版)[J]. 中华消化外科杂志, 2020, 19(6): 563-88.
[4]	中国医师协会结直肠肿瘤专业委员会, 中国抗癌协会大肠癌专业委员会, 国家癌症中心国家肿瘤质控中心结直肠癌质控专家委员会. 结直肠癌靶向治疗中国专家共识[J/CD]. 中华普通外科学文献（电子版）, 2023, 17(1): 1-8.
[5]	Margonis GA, Sergentanis TN, Ntanasis-Stathopoulos I, et al. Impact of surgical margin width on recurrence and overall survival following R0 hepatic resection of colorectal metastases: A systematic review and meta-analysis[J]. Ann Surg, 2018, 267(6): 1047-1055.
[6]	Hamady ZZ, Lodge JP, Welsh FK, et al. One-millimeter cancer-free margin is curative for colorectal liver metastases: A propensity score case-match approach[J]. Ann Surg, 2014, 259(3): 543-548.
[7]	Lauwerends LJ, van Driel P, Baatenburg de Jong RJ, et al. Real-time fluorescence imaging in intraoperative decision making for cancer surgery[J]. Lancet Oncol, 2021, 22(5): e186-e195.
[8]	Smith AM, Mancini MC, Nie S. Bioimaging: second window for in vivo imaging[J]. Nat Nanotechnol, 2009, 4(11): 710-711.
[9]	Goi T, Fujioka M, Satoh Y, et al. Angiogenesis and tumor proliferation/metastasis of human colorectal cancer cell line SW620 transfected with endocrine glands-derived-vascular endothelial growth factor, as a new angiogenic factor[J]. Cancer Res, 2004, 64(6): 1906-1910.
[10]	Freire Valls A, Knipper K, Giannakouri E, et al. VEGFR1+ metastasis-associated macrophages contribute to metastatic angiogenesis and influence colorectal cancer patient outcome[J]. Clin Cancer Res, 2019, 25(18): 5674-5685.
[11]	Boogerd L, Hoogstins C, Schaap DP, et al. Safety and effectiveness of SGM-101, a fluorescent antibody targeting carcinoembryonic antigen, for intraoperative detection of colorectal cancer: A dose-escalation pilot study[J]. Lancet Gastroenterol Hepatol, 2018, 3(3): 181-191.
[12]	de Gooyer JM, Elekonawo F, Bremers A, et al. Multimodal CEA-targeted fluorescence and radioguided cytoreductive surgery for peritoneal metastases of colorectal origin[J]. Nat Commun, 2022, 13(1): 2621.
[13]	Li F, Zhang Z, Cai J, et al. Primary preclinical and clinical evaluation of (68)Ga-DOTA-TMVP1 as a novel VEGFR-3 PET imaging radiotracer in gynecological cancer[J]. Clin Cancer Res, 2020, 26(6): 1318-1326.
[14]	Fleetwood F, Klint S, Hanze M, et al. Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic affibody molecules results in dramatically improved affinity[J]. Sci Rep, 2014, 4: 7518.
[15]	Hu Z, Fang C, Li B, et al. First-in-human liver-tumour surgery guided by multispectral fluorescence imaging in the visible and near-infrared-Ⅰ/Ⅱ windows[J]. Nat Biomed Eng, 2020, 4(3): 259-271.

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