Expression of aquaporin 3 in different states of small intestine and its clinical significance

doi:10.3877/cma.j.issn.1674-0793.2023.05.005

Abstract

Abstract:

Objective

To study the expression of aquaporin 3 (AQP3) in obstructive small intestinal tissues and analyze its significance in clinical practice.

Methods

Pathological sections, hematoxylin eosin staining (HE), and AQP3 immunohistochemical staining were performed in 25 normal small intestine, 25 hyperemic edema and 25 necrotic small intestine, to observe the structural changes and AQP3 expression status.

Results

The expression of AQP3 was strongly positive in normal small intestine. With the progression of small intestinal obstruction, intestinal congestion and edema, fibrous tissue hyperplasia, and vascular dilatation occurred. In parallel, the expression of AQP3 was significantly decreased in hyperemic edema small intestine compared to normal small intestine (P<0.001). AQP3 showed no expression in necrotic small intestine due to the destruction of small intestine, when progressing to the necrotic stage. The small intestinal obstruction patients with decreased AQP3 expression tended to have higher systemic inflammation than those with high AQP3 expression (P<0.05).

Conclusion

As the degree of necrosis worsens, the expression of AQP3 decreases in a gradient manner, suggesting that AQP3 may be a reliable marker for predicting necrosis of the obstructed small intestine.

Key words: Intestinal obstruction, Aquaporin 3, Necrosis, Inflammatory reaction

Haijian Zhao, Xin Zhao, Ning Chen, Jian Wang, Lun Zhu, Xiaoyu Zhang, Heng Li. Expression of aquaporin 3 in different states of small intestine and its clinical significance[J]. Chinese Archives of General Surgery(Electronic Edition), 2023, 17(05): 342-345.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2023.05.005

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2023/V17/I05/342

Figures/Tables 10

References 15

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组别	n	AQP3染色强度				Kruskal-Wallis P值	ANCOVA P值
组别	n	0	1	2	3	Kruskal-Wallis P值	ANCOVA P值
正常组	25	0	1	5	19
充血水肿组	25	1	10	8	6	<0.000 1	<0.000 1
坏死组	25	25	0	0	0

组别	n	AQP3染色强度				Kruskal-Wallis P值	ANCOVA P值
组别	n	0	1	2	3	Kruskal-Wallis P值	ANCOVA P值
正常组	25	0	1	5	19
充血水肿组	25	1	10	8	6	<0.000 1	<0.000 1
坏死组	25	25	0	0	0

项目	正常组				充血水肿组				P 1值
项目	全组	AQP3高表达	AQP3低表达	P值	全组	AQP3高表达	AQP3低表达	P值	P 1值
例数	25	19	6		25	6	19
降钙素原(μg/L)	0.40±0.65	0.44±0.73	0.27±0.21	0.401	1.56±3.49	0.38±0.75	1.95±3.95	0.067	0.122
白细胞计数(×10⁹/L)	8.17±2.82	8.63±2.94	6.70±1.95	0.226	12.04±5.45	11.42±3.27	12.24±6.04	0.424	0.002
中性粒细胞百分比(%)	73.67±8.76	74.57±9.15	70.83±7.35	0.645	82.48±11.19	79.33±13.47	83.47±10.59	0.098	0.003
血小板计数(×10⁹/L)	270.48±94.94	276.42±101.81	251.67±72.96	0.559	207.36±80.43	211.00±62.66	206.21±86.76	0.771	0.017
C反应蛋白(mg/L)	30.35±37.43	31.21±38.62	27.63±36.62	0.467	74.60±88.15	39.67±48.28	85.63±95.80	0.123	0.021

项目	正常组				充血水肿组				P 1值
项目	全组	AQP3高表达	AQP3低表达	P值	全组	AQP3高表达	AQP3低表达	P值	P 1值
例数	25	19	6		25	6	19
降钙素原(μg/L)	0.40±0.65	0.44±0.73	0.27±0.21	0.401	1.56±3.49	0.38±0.75	1.95±3.95	0.067	0.122
白细胞计数(×10⁹/L)	8.17±2.82	8.63±2.94	6.70±1.95	0.226	12.04±5.45	11.42±3.27	12.24±6.04	0.424	0.002
中性粒细胞百分比(%)	73.67±8.76	74.57±9.15	70.83±7.35	0.645	82.48±11.19	79.33±13.47	83.47±10.59	0.098	0.003
血小板计数(×10⁹/L)	270.48±94.94	276.42±101.81	251.67±72.96	0.559	207.36±80.43	211.00±62.66	206.21±86.76	0.771	0.017
C反应蛋白(mg/L)	30.35±37.43	31.21±38.62	27.63±36.62	0.467	74.60±88.15	39.67±48.28	85.63±95.80	0.123	0.021

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