LINC00520 promoting the invasion and metastasis of gastric cancer through miR-519b-3p/HIF1A axis

doi:10.3877/cma.j.issn.1674-0793.2024.06.006

Abstract

Abstract:

Objective

To investigate the function and molecular mechanism of LINC00520 in gastric cancer and to reveal the potential role of LINC00520 in gastric cancer development.

Methods

GEO database were screened for differentially expressed genes associated with malignant progression of gastric cancer. qPCR was performed to detect the expression of LINC00520 in gastric cancer， and cellular function experiments were performed to explore the potential effects of LINC00520. The association between LINC00520 and miR-519b-3p and HIF1A was detected by bioinformatics analysis， and their binding activities were verified by using dual luciferase reporter gene and RNA immunoprecipitation assays.

Results

In gastric cancer， the expression of LINC00520 was significantly increased （P＜0.01）， and LINC00520 promoted the malignant progression of gastric cancer cells （P＜0.01）. By bioinformatics， dual luciferase reporter gene and RNA immunoprecipitation assays， it was found that LINC00520 upregulated HIF1A expression by competitively targeting miR-519b-3p （P＜0.01）.

Conclusion

High expression of LINC00520 in gastric cancer plays a critical role by targeting the miR-519b-3p/HIF1A axis， which provides new targets for the diagnosis and treatment of gastric cancer.

Key words: LINC00520, Stomach neoplasms, RNA， long noncoding, miR-519b-3p, HIF1A；ceRNA

Jie An, Yunfeng Niu, Wei Liu. LINC00520 promoting the invasion and metastasis of gastric cancer through miR-519b-3p/HIF1A axis[J]. Chinese Archives of General Surgery(Electronic Edition), 2024, 18(06): 430-436.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2024.06.006

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2024/V18/I06/430

Figures/Tables 9

References 16

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基因	引物序列	退火温度（℃）	产物大小（bp）
LINC00520	F：5'-GCAGTTGAGCTTCTTGTGCC-3'	60	250
	R：5'-GTGGTCAGAGGTGGCCATAC-3'
HIF1A	F：5'-GCCCTAACGTGTTATCTGTCG-3'	58	248
	R：5'-CTTTTGCTCCATTCCATTCTG-3'
GAPDH	F：5'-AGGTGAAGGTCGGAGTCAACG-3'	58	102
	R：5'-AGGGGTCATTGATGGCAACA-3'

基因	引物序列	退火温度（℃）	产物大小（bp）
LINC00520	F：5'-GCAGTTGAGCTTCTTGTGCC-3'	60	250
	R：5'-GTGGTCAGAGGTGGCCATAC-3'
HIF1A	F：5'-GCCCTAACGTGTTATCTGTCG-3'	58	248
	R：5'-CTTTTGCTCCATTCCATTCTG-3'
GAPDH	F：5'-AGGTGAAGGTCGGAGTCAACG-3'	58	102
	R：5'-AGGGGTCATTGATGGCAACA-3'

位点	正义链序列	反义链序列
372	GAATAGTAGTAGTTAACAA	TTGTTAACTACTACTATTC
542	GGATGCAGAAGTACTCATA	TATGAGTACTTCTGCATCC
708	GGACTGCAATTCACAAGAA	TTCTTGTGAATTGCAGTCC
1344	GATTCTACAGCTCAGCAAA	TTTGCTGAGCTGTAGAATC

位点	正义链序列	反义链序列
372	GAATAGTAGTAGTTAACAA	TTGTTAACTACTACTATTC
542	GGATGCAGAAGTACTCATA	TATGAGTACTTCTGCATCC
708	GGACTGCAATTCACAAGAA	TTCTTGTGAATTGCAGTCC
1344	GATTCTACAGCTCAGCAAA	TTTGCTGAGCTGTAGAATC

项目	n	LINC00520			miR-519b-3p			HIF1A
项目	n	表达量	t值	P值	表达量	t值	P值	表达量	t值	P值
年龄（岁）			-0.008	0.996		0.610	0.545		-0.463	0.644
≤60	24	2.263±1.287			0.631±0.376			2.162±1.338
＞60	43	2.265±1.443			0.583±0.266			2.313±1.241
性别			0.600	0.551		-0.897	0.372		0.830	0.409
男	47	2.331±1.530			0.578±0.299			2.343±1.275
女	20	2.109±0.953			0.652±0.329			2.061±1.264
淋巴结转移			-2.719	0.008		4.725	＜0.001		-4.080	＜0.001
无	21	1.617±0.826			0.829±0.275			1.417±0.888
有	46	2.560±1.484			0.496±0.264			2.643±1.236
组织分化程度			-0.017	0.986		-0.715	0.481		0.204	0.839
中、高	38	2.262±1.395			0.577±0.273			2.286±1.151
低	29	2.268±1.383			0.631±0.351			2.222±1.428
TNM分期			-0.330	0.743		0.792	0.427		-1.401	0.166
Ⅰ+Ⅱ	18	2.172±1.115			0.650±0.251			1.903±1.327
Ⅲ+Ⅳ	49	2.298±1.474			0.582±0.327			2.389±1.235

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