Abstract:
Objective
To investigate the role of K-ras，P53，and P16 gene of duodenal juice detection in diagnosis of biliary tract carcinoma （BTC）.
Methods
The K-ras，P53 and P16 gene mutations in the bile and duodenal juice from 30 cases with BTC and 10 cases with benign choledocholithiasis were detected by PCR-SSCP-AgNO3 staining or PCR-RFLP.
Results
The positive rates of K-ras，P53 and P16 gene mutations and combination assay with them in bile supernatant of patients with BTC were 56%，50%，33% and 83.3%，respectively.The positive rates of them in duodenal juice were 43%，36%，20% and 70%，respectively.However，K-ras gene mutation was detected in only one case with benign choledochlithiasis.Statistic analysis show there was significant difference in those gene mutations between BTC group and benign group （P＜0.001）.Positive rates of K-ras，P53 and P16 gene mutations were higher in the supernatant than that in the sediment （P＜0.001）.For the negative of blie duct brushing in BTC，positive rate of combination assay with these gene mutations was still high （66.67%）.
Conclusions
Combined assay of K-ras，P53 and P16 gene mutations in duodenal juice might be useful for diagnosis of BTC.
Key words:
Biliary tract carcinoma,
K-ras,
P53,
P16,
duodenal juice
Ping XUE, Qiang ZHENG, Hai-wu LU, Yi-ze HU. Clinical singnificance in diagnosis of biliary tract carcinoma by detection of K-ras、P53 and P16 gene mutations in duodenal juice[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(01): 36-39.