Home    中文  
 
  • Search
  • lucene Search
  • Citation
  • Fig/Tab
  • Adv Search
Just Accepted  |  Current Issue  |  Archive  |  Featured Articles  |  Most Read  |  Most Download  |  Most Cited

Chinese Archives of General Surgery(Electronic Edition) ›› 2008, Vol. 02 ›› Issue (05): 358-362. doi: 10.3877/cma.j.issn.1674-0793.2008.05.007

• Original articals • Previous Articles     Next Articles

Velcade induced apoptosis in HepG2 cells

Xi-lin CHEN1, Qian WANG,1(), Sui JIANG1, Liang-qi CAO1, Xiao-hui HUANG1, Hao-xiang TAN1, Jing-song CHEN1   

  1. 1.Department of General Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080,China
  • Received:2008-07-20 Online:2008-10-01 Published:2024-11-29
  • Contact: Qian WANG

Abstract:

Objective

To investigate the effects of Velcade,a proteasome inhibitor,on HepG2 cells,and to explore the mechanism.

Methods

HepG2 cells were treated with various concentrations of Velcade for 24 h and 48 h. The cell viability was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)assay.Cell apoptosis was investigated by flow cytometry.Western blot assayed the change of caspase 3.The expressions of Bcl-2 family,Cyclin A and Cyclin D were detected by reverse transcription-polymerase chain reaction (RT-PCR).

Results

Viabilities of HepG2 cells treated with various concentrations of Velcade(32,64,128,256,512 nmol/L)for 48 h were significantly decreased compared to control group.Viabilities of HepG2 cells gradually reduced to 91.4%±2.1%, 75.0%±3.7%, 57.3%±1.6%, 52.7%±1.6% and 31.4%±2.6%, respectively. The differences compared with control group (100.0%±1.7%)were significant (P<0.05).The percent of SubG1 of HepG2 cells (27.3%±5.3%)treated with 128 nmol/L Velcade 48 h was significantly higher than that in control group(4.3%±0.5%,P<0.05).After treatment with Velcade(128 nmol/L)for 24 h,the expression of caspase 3 markedly decreased compared with control. Velcade greatly increased the G2/M phase in HepG2 cells. Velcade resulted in no significant changes of Bcl-2 family member.However,Velcade down regulated the expressions of Cyclin A and Cyclin D.

Conclusion

The mechanism of Velcade to induced apoptosis in HepG2 is not associated with change of Bcl-2 family member.Velcade induced G2/M phase arrest via down regulation of Cyclin A and D.

Key words: Proteasome inhibitor, Velcade, Hepatocellular carcinoma, Apoptosis

京ICP 备07035254号-20
Copyright © Chinese Archives of General Surgery(Electronic Edition), All Rights Reserved.
Tel: 020-87331056 E-mail: pwwxcma2007@126.com
Powered by Beijing Magtech Co. Ltd