Abstract:
Objective To observe the effect of denervation on wound healing rate, cell proliferation activity and vascular regeneration.
Methods Right spinal nerves of T9 to L1 of were isolated and dissected on forty Spraque-Dawley(SD) rats. Subsequently, 1.0cm-diameter round full-thickness cutaneous wounds were made symmetrically within the denervated area on the right side of the back(experimental group) and the normal innervated area on the left side(control group). Wounds were measured and harvested at 1、2、3、4 weeks. Wound healing rate, histology and morphometry, cell proliferation activity and vascular regeneration were analyzed over time.
Results The wounds of control group and experimental group were completely heal on 3rd and 4th week respectively. Epithelization of control group appeared earlier and faster than experimental group, wound healing rate of experimental group were decreased significantly at 2nd and 3rd week (P<0.05) as well. The cell proliferation activity of wounds surface were higher in dermis and epidermis. Furthermore, cell proliferation activity of experimental group were weaker than control group during 2 to 4 weeks (P<0.05) . Regenerated wound capillary vessel laied in dermis mainly. Wound capillary vessel density was lower significantly in the experimental group (P<0.05) at 2 to 4 weeks when compared with the controls.
Conclusion Loss of innervation on skin may be responsible for the cell proliferation activity hypoproliferation, wound healing delay and vascular regeneration depression.
Key words:
Denervation,
Wound Surface,
Vascular Regeneration
Bin SHU, Shao-hai QI, Yong HUANG, Wen LAI, Ren-xiang MAO, Ju-lin XIE, Ying-bin XU, Xu-sheng LIU, Xia LI. Effect of denervation on skin wound healing in rats[J]. Chinese Archives of General Surgery(Electronic Edition), 2010, 04(02): 100-104.