Effects of gender hormones on proliferation and apoptosis of IEC-6 cells after cutaneous burn

doi:10.3877/cma.j.issn.1674-0793.2011.01.008

Abstract

Abstract:

Objective

To explore the effects of sex steroids on post-burn intestinal epithelial cells.

Methods

Sera from SD rats which received 30% total-body-surface full-thickness burn or sham treatment were used to treat confluent monolayer of IEC-6 cells. Diethylstilbestrol (DES), testosterone (T), or vehicle were then added. the effects on proliferation, cell-cycle distribution, apoptosis, cell number and Claudin-1 expression were investigated by means of MTT assays, flow cytometry, DAPI staining, RT-CES and fluorescent immunoassay, respectively.

Results

Serum from burned animals enhanced proliferation (0.777 vs 0.415, P<0.001), apoptosis (10.5% vs 1.59%, P<0.001) and Claudin-1 expression of IEC-6 cells, while reduced the cell number. Both DES and T inhibited proliferation and apoptosis of cells treated by scald serum. Specifically, proliferation index (12.3% vs 14.6%, P<0.001) and apoptotic rate (1.04% vs 2.33%, P<0.005) were lower in cells treated by DES than cells treated by T.

Conclusions

Post-burn intestinal epithelial barrier dysfunction is mainly due to surpass of apoptosis over proliferation. Both gender hormones inhibit proliferation and apoptosis in intestinal epithelial cells caused by cutaneous burn. In addition, estrogen had more obvious effects than androgen.

Key words: Burn, Diethylstilbestrol, Testosterone, IEC-6 cell

Guan-qing SUN, Zheng-hua REN, Ze-ren HUO, Ting YANG, Sheng LI, Han-ping SHI, Xiang-dong GUAN. Effects of gender hormones on proliferation and apoptosis of IEC-6 cells after cutaneous burn[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(01): 28-34.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2011.01.008

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2011/V05/I01/28

Figures/Tables 6

References 24

1	Choudhry MA, Rana SN, Kavanaugh MJ, et al. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury. Alcohol, 2004, 33(3): 199-208.
2	Al-Ghoul WM, Khan M, Fazal N, et al. Mechanisms of postburn intestinal barrier dysfunction in the rat: roles of epithelial cell renewal, E-cadherin, and neutrophil extravasation. Crit Care Med, 2004, 32(8): 1730-1739.
3	宋国栋, 王德昌, 贾军, 等. 重组人生长激素对烧伤大鼠肠粘膜结构及细胞凋亡的影响. 中华烧伤杂志, 2002, 18(4): 207-209.
4	赵云, 王凤君, 王裴, 等. 胰高血糖素样肽-2对烧伤大鼠肠粘膜细胞增殖的影响. 中华烧伤杂志, 2003, 19(4): 209-212.
5	Varedi M, Greeley GH, Herndon DN, et al. A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration. Am J Physiol Gastrointest Liver Physiol, 1999, 277(1): G175-182.
6	Kovacs EJ. Aging, traumatic injury, and estrogen treatment. Exp Gerontol, 2005, 40(7): 549-555.
7	Horton JW, White DJ, Maass DL. Gender-related differences in myocardial inflammatory and contractile responses to major burn trauma. Am J Physiol Heart Circ Physiol, 2004, 286(1): H202-213.
8	Ozveri ES, Bozkurt A, Haklar G, et al. Estrogens ameliorate remote organ inflammation induced by burn injury in rats. Inflamm Res, 2001, 50(12):585-591.
9	Di Domenico M, Castoria G, Bilancio A, et al. Estradiol activation of human colon carcinoma-derived Caco-2 cell growth. Cancer Res, 1996, 56(19):4516-4521.
10	Booth C, Hargreaves DF, Hadfield JA, et al. Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro. Br J Cancer, 1999, 80(10): 1550-1557.
11	Wright NA, Morley AR. The effect of testosterone on the growth fraction of the mouse small intestine. J Endocrinol, 1971, 50(2): 351-352.
12	Quaroni A, Wands J, Trelstad RL, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J Cell Biol, 1979, 80(2): 248-265.
13	Spies M, Chappell VL, Dasu MR, et al. Role of TNF-α in gut mucosal changes after severe burn. Am J Physiol Gastrointest Liver Physiol, 2002, 283(4): G703-708.
14	Wolf SE, Ikeda H, Matin S, et al. Cutaneous burn increases apoptosis in the gut epithelium of mice. J Am Coll Surg, 1999, 188(1): 10-16.
15	Huang Y, Zheng J, Fan P, et al. Transfection of antisense p38 alpha gene ameliorates myocardial cell injury mediated by hypoxia and burn serum. Burns, 2007, 33(5): 599-605.
16	Wu X, Woodside KJ, Song J, et al. Burn-induced gut mucosal homeostasis in TCR delta receptor-deficient mice. Shock, 2004, 21(1): 52-57.
17	Abreu MT, Arnold ET, Chow JY, et al. Phosphatidylinositol 3-kinase-dependent pathways oppose Fas-induced apoptosis and limit chloride secretion in human intestinal epithelial cells. Implications for inflammatory diarrheal states. J Biol Chem, 2001, 276(50): 47 563-47 574.
18	Jeschke MG, Bolder U, Chung DH, et al. Gut mucosal homeostasis and cellular mediators after severe thermal trauma and the effect of insulin-like growth factor-I in combination with insulin-like growth factor binding protein-3. Endocrinology, 2007, 148(1): 354-362.
19	Yu HP, Hsieh YC, Suzuki T, et al. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway. J Leukoc Biol, 2007, 82(1): 1-7.
20	Krajewski S, Krajewska M, Shabaik A, et al. Immunohistochemical determination of In vivo distribution of bax, a dominant inhibitor of bcI-2. Am J Pathol, 1994, 145(6): 1323-1336.
21	Shima Y, Tajiri T, Taguchi T, et al. Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury: a possible correlation with the proliferation or apoptosis of intestinal epithelial cells. J Pediatr Surg, 2006, 41(4): 830-836.
22	Song J, Wolf SE, Herndon DN, et al. Second hit post burn increased proximal gut mucosa epithelial cells damage. Shock, 2008, 30(2): 184-188.
23	Saegusa M, Takano Y, Okayasu I. Bcl-2 expression and its association with cell kinetics in human gastric carcinomas and intestinal metaplasia. J Cancer Res Clin Oncol, 1995, 121(6): 357-363.
24	Clark J, Saunders T, Doelle S, et al. Tight junctions formation in experimental model of necrotizing enterocolitis-effect of EGF treatment. J Pediatr Gastroenterol Nutr, 2005, 40(5): 617.

[1]	Xiaojie He, Leilei Zheng, Hanghui Cen, Chunmao Han. A follow-up study of post-traumatic stress disorder in severe burn patients [J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2023, 16(02): 111-115.
[2]	Jiajin Tu, Wuqiang Liao, Jinjing Liu, Zhipeng Tu, Yuangui Mao. Risk factors and prognosis analysis of Acinetobacter baumannii bloodstream infection in patients with severe burns [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 491-497.
[3]	Cheng Wang, Huijun Zhang, Fengjun Qin, Hui Chen. Comparation of efficacy on meshed skin grafting and ReCell epidermal cell transplantation in the treatment of deep second degree burns [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(06): 498-502.
[4]	Pediatric Burn Study Group, Burn Surgery Branch of Chinese Medical Association. Expert consensus on fluid resuscitation of early pediatric burn shock (version 2023) [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(05): 371-376.
[5]	Roumei Cai, Jiemei Zeng, Weili Huang, Wenmin Xie, Yandan Liu, Manjun Wu, Chuyan Cai. Clinical research of QC circle intervention to reduce the incidence of catheter related infection during femoral vein catheterization in burn wounds [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(05): 399-404.
[6]	Wei Yang, Xia Hao, Dongzhen Zhu, Jinbo Zhang, Xuefei Tian, Bin Yao. A meta analysis of the clinical effect of traditional Chinese medicine on burn and scald patients [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(05): 419-426.
[7]	Siyan Xia, Shizhao Ji, Wanfang Zhou, Runzhi Huang, Lijun Zhang. Survey of the readiness for hospital discharge and its influencing factors in burn and trauma patients [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(04): 331-337.
[8]	Tianfeng Ru, Haonan Dai, Lin Yuan, Kunping Wu, Weiguo Xie. Observation of application for muscle ultrasound in the assessment of muscle performance in severely burned patients [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(03): 197-203.
[9]	Jiao Yang, Ling Wang, Lan Gu, Shentong An, Dahai Hu, Juntao Han. Effect of esmketamine on venipuncture in children undergoing burn scar surgery [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(03): 210-216.
[10]	Feng Li, Junyou Li, Shutang Feng, Guoping Li, Jierong Yang. Clinical observation of skin xenotransplantation using GGTA1/β4GalNT2 double gene knockout inbred line Wuzhishan miniature pig [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(03): 241-248.
[11]	Xiaobin Liu, Feng Zhu. Pharmacological treatments in critical burn patients with augmented renal clearance [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(03): 260-264.
[12]	Dongjie Li, Xiaoming Jia. Research progress on skin preservation and cryoinjury in burn surgery [J]. Chinese Journal of Injury Repair and Wound Healing(Electronic Edition), 2023, 18(02): 93-97.
[13]	Ning Mao, Wenting Xu, Yong Zhu. Relationship between androgen levels and perianal abscesses: a Mendelian randomization study [J]. Chinese Journal of Colorectal Diseases(Electronic Edition), 2023, 12(06): 490-494.
[14]	Yan Wang, Rui Guo, Shujie Wang. Construction of a death risk diagnosis model in elderly burn patients after tracheotomy and analysis of countermeasures [J]. Chinese Journal of Diagnostics(Electronic Edition), 2023, 11(04): 249-253.
[15]	Lei Gao, Quan Li, Yaliga Ba, Qiang Chen, Zhihui Hou, Shengjun Cao, Te Ba. Effects of platelet exosomes on coagulation function in severe burn patients: a preliminary study [J]. Chinese Journal of Hygiene Rescue(Electronic Edition), 2023, 09(03): 149-154.

Please choose a citation manager

Content to export