Expression difference of Livin mRNA in colorectal carcinoma, adenoma, and normal colorectal mucosa and its clinical significance

doi:10.3877/cma.j.issn.1674-0793.2011.03.004

Abstract

Abstract:

Objective

To compared the difference of Livin mRNA expression among colorectal adenocarcinoma, colorectal adenoma and normal mucosa, to further investigate the potential role of Livin in carcinogenesis.

Methods

The expressions of Livin mRNA were determined by real-time RT-PCR in tissues of 12 colorectal normal mucosa, 36 colorectal adenomas and 64 colorectal carcinomas. Date were evaluated by relative expression software tool (REST-XL^?).

Results

The expression of Livin mRNA elevated in turn by colorectal normal mucosa, adenoma and adenocarcinoma. The expression of Livin mRNA in colorectal carcinomas was significantly higher than that in adenomas, as well as significantly higher than that in normal mucosa (P= 0.001), while the diffence between adenoma and normal mucosa was not significant (P=0.19).

Conclusions

The difference in expression of Livin between adenoma and adenocarcinoma indicates that Livin might play an important role in carcinogenesis of colorectal cancer.

Key words: Colorectal neoplasms, Inhibitor of apoptosis proteins, Livin, Real-time reverse transcription-PCR

Yong WANG, Yi-cai SONG, Zuo-wen YIN, Jun YU, Jun-hui LIU, Yi-fan LI, Mei-mei GUO. Expression difference of Livin mRNA in colorectal carcinoma, adenoma, and normal colorectal mucosa and its clinical significance[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(03): 195-197.

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References 13

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标本来源	n	Livin平均Ct值	β-actin平均Ct值
正常结直肠黏膜	12	34.145	24.591
结直肠腺瘤	36	31.632	23.900
结直肠癌	64	28.215^*#	24.122

标本来源	n	Livin平均Ct值	β-actin平均Ct值
正常结直肠黏膜	12	34.145	24.591
结直肠腺瘤	36	31.632	23.900
结直肠癌	64	28.215^*#	24.122

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