Expression and clinical significance of YKL-40 protein in human splenomegaly of portal hypertension

doi:10.3877/cma.j.issn.1647-0793.2013.02.002

Abstract

Abstract:

Objective

To investigate the expression and clinical significance of YKL-40 protein in human splenomegaly tissue of portal hypertension.

Methods

Immunohistochemical method was used to detect the expression of YKL-40 in spleen of portal hypertension. Masson trichrome staining method was used to detect the fibrosis degrees of spleens associated with portal hypertension.

Results

Expression of YKL-40 in enlarged spleens of portal hypertension was significantly higher than that in normal spleen (P < 0.05), and its expression was significantly associated with Child-Pugh Classification (P < 0.05). Furthermore, the statistical correlation between overexpression of YKL-40 and Free Portal Pressure (FPP) was found(R = 0.499, P < 0.01). Masson trichrome staining showed that a considerable amount of fibrosis extending to the entire splenic parenchyma was found in portal hypertension patients, compared with normal people(P < 0.05). Additionally, expression of YKL-40 was correlated with fibrosis area of spleen (correlation coefficient was 0.857, P < 0.01).

Conclusions

Expression of YKL-40 in enlarged spleens of portal hypertension was significantly higher than that in normal spleen, and statistical correlation between YKL-40 with FPP and Child-Pugh Classification are found. The expression of YKL-40 is correlated with fibrosis area of spleen. YKL-40 may play an important role in molecular mechanism involved in fibrosis development of splenomegaly associated with portal hypertension.

Key words: YKL-40, Splenomegaly, Portal hypertension, Fibrosis

Dong WANG, Xi-lin DU, Rui DONG, Ji-kai YIN, Li ZANG, Xue JIANG, Jian-guo LU. Expression and clinical significance of YKL-40 protein in human splenomegaly of portal hypertension[J]. Chinese Archives of General Surgery(Electronic Edition), 2013, 07(02): 86-90.

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Figures/Tables 5

References 12

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?	YKL-40阳性组（n=33）	YKL-40阴性组（n=15）	P值
年龄(岁)	45(22~69)	40(19~58)	0.310
红细胞(×10¹²/L)	3.59(2.64~5.02)	3.41(2.57~3.90)	0.059
白细胞(×10⁹/L)	1.68(0.10~7.04)	2.02(0.77~3.11)	0.771
血小板(×10⁹/L)	43(20~119)	50(11~94)	0.900
血清白蛋白(g/L)	31.7(25.8~41.8)	33.6(27.0~38.7)	0.789
血清TBIL (μmol/L)	39.60(21.40~74.50)	33.08(15.20~51.20)	0.045^a
血清GPT (U/L)	27(9~75)	21(11~61)	0.051
血清GOT (U/L)	40(17~110)	30(17~74)	0.016 ^a

?	YKL-40阳性组（n=33）	YKL-40阴性组（n=15）	P值
年龄(岁)	45(22~69)	40(19~58)	0.310
红细胞(×10¹²/L)	3.59(2.64~5.02)	3.41(2.57~3.90)	0.059
白细胞(×10⁹/L)	1.68(0.10~7.04)	2.02(0.77~3.11)	0.771
血小板(×10⁹/L)	43(20~119)	50(11~94)	0.900
血清白蛋白(g/L)	31.7(25.8~41.8)	33.6(27.0~38.7)	0.789
血清TBIL (μmol/L)	39.60(21.40~74.50)	33.08(15.20~51.20)	0.045^a
血清GPT (U/L)	27(9~75)	21(11~61)	0.051
血清GOT (U/L)	40(17~110)	30(17~74)	0.016 ^a

临床病理参数		例数	YKL-40阳性[n/n(%)]	P值
门静脉高压脾脏		48	33/48(68.8)	?
年龄(岁)		?	?	0.108
?	≤44	27	16/27(59.3)	?
?	>44	21	17/21(81.0)	?
性别		?	?	0.585
?	男	33	24/33(72.7)	?
?	女	15	9/15(60.0)	?
食管静脉曲张程度		?	?	0.405
?	无	3	1/3(33.3)	?
?	轻微	9	6/9(66.7)	?
?	中、重度	36	26/36(72.2)	?
Child-Pugh分级		?		0.000
?	A	14	3/14(21.4)	?
?	B	26	22/26(84.6)	?
?	C	8	8/8(100.0)	?

临床病理参数		例数	YKL-40阳性[n/n(%)]	P值
门静脉高压脾脏		48	33/48(68.8)	?
年龄(岁)		?	?	0.108
?	≤44	27	16/27(59.3)	?
?	>44	21	17/21(81.0)	?
性别		?	?	0.585
?	男	33	24/33(72.7)	?
?	女	15	9/15(60.0)	?
食管静脉曲张程度		?	?	0.405
?	无	3	1/3(33.3)	?
?	轻微	9	6/9(66.7)	?
?	中、重度	36	26/36(72.2)	?
Child-Pugh分级		?		0.000
?	A	14	3/14(21.4)	?
?	B	26	22/26(84.6)	?
?	C	8	8/8(100.0)	?

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