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Chinese Archives of General Surgery(Electronic Edition) ›› 2013, Vol. 07 ›› Issue (02): 96-100. doi: 10.3877/cma.j.issn.1647-0793.2013.02.004

Special Issue:

• Original Article • Previous Articles     Next Articles

Site-directed analysis of key transcriptional regulatory regions of human integrin beta6 gene in HepG2 cells

Rui-rui XU1, Hui HAN1, Wen-jing HE1, Geng-zhen CHEN1,()   

  1. 1. Minimally Invasive Medical Center, the Second Affliated Hospital of Shantou University Medical College, Shantou, 515041, China
  • Received:2012-12-17 Online:2013-04-01 Published:2013-04-01
  • Contact: Geng-zhen CHEN
  • About author:
    Corresponding author: CHEN Geng-zhen, Email:

Abstract:

Objective

To identify key transcriptional regulatory regions of human integrin beta6 (Itgβ6) gene in HepG2 cells, thus to make a preliminary study for its transcriptional regulatory mechanism.

Methods

The recombinant plasmids containing different lengths of DNA fragments upstream of translation initiation site of Itgβ6 gene as reporter promoters were constructed by deletion Method, and the promoter activities were detected using dual luciferase reporter assay system. The potential transcription factor binding sites at key transcriptional regulatory region of Itgβ6 gene were predicted by TESS.

Results

The recombinant plasmids containing different lengths of Itgβ6 gene were obtained. When the lengths of Itgβ6 gene were reduced from -458 bp to -187 bp, the transcriptional activity was decreased by about 50% . There were YY1、AP-1 and c-Myb transcriptional factor binding sites in the -458 bp to -187 bp regions.

Conclusion

The -458 bp/ -187 bp was the key transcriptional regulatory region of Itgβ6 gene in HepG2 cells, YY1、AP-1 and c-Myb are in this region, and may be the important factors for regulating the transcription of Itgβ6 gene.

Key words: Integrin beta6, Recombinant plasmid, Hepatocellular carcinoma, Transcriptional factor

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