Relationship between the hematological outcomes of splenectomy and the expression of Th cytokines of peripheral blood in ITP patients

doi:10.3877/cma.j.issn.1674-0793.2014.02.008

Abstract

Abstract:

Objective

To investigate the relationship between the hematological outcomes of splenectomy and the mRNA expressions of Th cytokines in primary immune thrombocytopenia (ITP) patients.

Methods

The mRNA expression of Th1 (IL-2, IFN-γ), Th2 (IL-4, IL-5, IL-6, IL-10), Th3 (TGF-β1), and Th17 (IL-17) cytokines in peripheral blood before and after laparoscopic splenectomy were detected by the QuantiGene Plex method from fourteen cases of responder group (Group R) and eight cases of non-responder group (Group NR). Thirty healthy subjects were served as the control group.

Results

TGF-β1 mRNA expressions before and after splenectomy in Group R were significantly higher than those in Group NR (P values were 0.001 and 0.006, respectively) and control group (P values were 0.001 and <0.001, respectively). No significant differences of IL-2, IFN-γ and IL-17 mRNA expression before or after splenectomy between Group R and Group NR were observed.

Conclusions

There are different TGF-β1 mRNA expressions in ITP patients with different responses to splenectomy. Preoperative detection of TGF-β1 expression is helpful to predict the outcome of surgery.

Key words: Immune thrombocytopenia, Splenectomy, Cytokines

Dong Zheng, Longjuan Zhang, Qian Wang, Jun Wang, Chensong Huang, Chaoxu Zheng. Relationship between the hematological outcomes of splenectomy and the expression of Th cytokines of peripheral blood in ITP patients[J]. Chinese Archives of General Surgery(Electronic Edition), 2014, 08(02): 116-120.

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Figures/Tables 4

References 13

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探针编号	标志物	序列长度	探针设置区域
NM_001101	β-Actin	1 852	1 164~1 762
NM_000942	PPIB	1 045	82~366
NM_000586	IL-2	822	58~625
NM_000619	IFN-γ	1 240	53~438
NM_000589	IL-4	921	2~585
NM_000879	IL-5	816	19~662
NM_000600	IL-6	1 201	3~669
NM_000572	IL-10	1 629	44~654
NM_000660	TGF-β1	2 217	1 296~1 787
NM_002190	IL-17	1 859	980~1 731

探针编号	标志物	序列长度	探针设置区域
NM_001101	β-Actin	1 852	1 164~1 762
NM_000942	PPIB	1 045	82~366
NM_000586	IL-2	822	58~625
NM_000619	IFN-γ	1 240	53~438
NM_000589	IL-4	921	2~585
NM_000879	IL-5	816	19~662
NM_000600	IL-6	1 201	3~669
NM_000572	IL-10	1 629	44~654
NM_000660	TGF-β1	2 217	1 296~1 787
NM_002190	IL-17	1 859	980~1 731

?	例数	男/女（例）	年龄(岁)	病程(月)	随访时间(月)
手术有效组（R组）	14	4/10	37.86±4.94	30.50±8.91	21.57±9.15
手术无效组（NR组）	8	4/4	34.25±6.79	13.88±3.53	20.25±7.63
对照组	30	18/12	41.13±2.38	?	?
χ²/Z /t值	?	0.663	-0.390	-1.734	-0.345
P值	?	0.624	0.701	0.101	0.734

?	例数	男/女（例）	年龄(岁)	病程(月)	随访时间(月)
手术有效组（R组）	14	4/10	37.86±4.94	30.50±8.91	21.57±9.15
手术无效组（NR组）	8	4/4	34.25±6.79	13.88±3.53	20.25±7.63
对照组	30	18/12	41.13±2.38	?	?
χ²/Z /t值	?	0.663	-0.390	-1.734	-0.345
P值	?	0.624	0.701	0.101	0.734

组别	例数	TGF-β1	IL-17	IL-2	IFN-γ
手术有效组（R组）	14	178.27±14.15^ab	53.15±11.75^b	7.54±2.23^b	6.59±1.20
手术无效组（NR组）	8	118.76±11.81	48.36±12.90^b	4.28±1.80^b	5.23±1.03
对照组	30	134.16±5.84	17.07±4.28	31.77±3.75	5.66±0.51

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