Expression of vascular endothelial growth factor-C protein and mRNA in gastric carcinoma and its relationship with lymph node micrometastases

doi:10.3877/cma.j.issn.1674-0793.2014.05.013

Abstract

Abstract:

Objective

To explore the expression of vascular endothelial growth factor-C (VEGF-C) protein and mRNA in gastric cancer and its relationship with lymph node micrometastases.

Methods

Immunohistochemistry and RT PCR were used to detect the expression of VEGF-C protein and mRNA in gastric carcinoma tissue, adjacent tissue and normal tissue from 80 patients who suffered from gastric carcinoma. The expression level of VEGF-C protein and mRNA were compared between tissues with positive or negative lymph node micrometastases.

Results

The positive expression rates of VEGF-C protein and mRNA in gastric cancer tissue were significantly higher than those in adjacent tissue and normal tissue (P<0.05) , which had no relationship with the patients' age, sex, tumor size, location and distant metastasis. The expression of VEGF-C was positively correlated with lymph node metastasis (P<0.01) , and that of VEGF-C mRNA with micrometastasis was closer than that of protein.

Conclusions

VEGF-C protein and mRNA in gastric cancer tissue is correlated with lymph node micrometastases, and detection of VEGF-C mRNA is prior to detection of protein. VEGF-C mRNA can be serving as a more accurate indicator of lymph node micrometastases in the gastric cancer patients.

Key words: Gastric cancer, Vascular endothelial growth factor-C, mRNA, Lymph node micrometastases

Zhifeng Yang, Yuanlong Yu, Qingyan Deng. Expression of vascular endothelial growth factor-C protein and mRNA in gastric carcinoma and its relationship with lymph node micrometastases[J]. Chinese Archives of General Surgery(Electronic Edition), 2014, 08(05): 395-398.

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Figures/Tables 2

References 11

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淋巴结转移	例（%）	蛋白表达阳性	mRNA表达阳性
无	15（18.75）	6（40.00）	1（6.67）
微转移	23（28.75）	5（21.74）	19（82.61）
有	42（52.50）	28（66.67）	38（90.48）

淋巴结转移	例（%）	蛋白表达阳性	mRNA表达阳性
无	15（18.75）	6（40.00）	1（6.67）
微转移	23（28.75）	5（21.74）	19（82.61）
有	42（52.50）	28（66.67）	38（90.48）

项目		例数	VEGF-C mRNA表达		χ²值	P值
项目		例数	阳性（58例）	阴性（22例）	χ²值	P值
性别		?	?	?	?	?
?	男	53	35	18	3.289	0.111
?	女	27	23	4	?	?
年龄（岁）		?	?	?	?	?
?	≥55	47	34	13	0.001	1.000
?	<55	33	24	9	?	?
肿瘤直径（cm）		?	?	?	?	?
?	≤2	16	12	4	0.515	0.773
?	2～5	53	39	14	?	?
?	≥5	11	7	4	?	?
肿瘤位置		?	?	?	?	?
?	胃窦	32	24	8	0.496	0.780
?	胃底	5	3	2	?	?
?	胃体	43	31	12	?	?
组织分型		?	?	?	?	?
?	高分化腺癌	6	1	5	22.425	<0.001
?	中分化腺癌	7	2	5	?	?
?	低分化腺癌	45	40	5	?	?
?	印戒细胞癌	22	15	7	?	?
肿瘤浸润深度		?	?	?	?	?
?	T1	8	5	3	25.171	<0.001
?	T2	35	23	12	?	?
?	T3	21	16	5	?	?
?	T4	16	14	2	?	?
淋巴结转移		?	?	?	?	?
?	无	15	1	14	21.002a	<0.001 a
?	微转移	23	19	4	?	?
?	有	42	38	4	?	?
远处转移		?	?	?	?	?
?	无	70	51	19	0.036	1.000
?	有	10	7	3	?	?

项目		例数	VEGF-C mRNA表达		χ²值	P值
项目		例数	阳性（58例）	阴性（22例）	χ²值	P值
性别		?	?	?	?	?
?	男	53	35	18	3.289	0.111
?	女	27	23	4	?	?
年龄（岁）		?	?	?	?	?
?	≥55	47	34	13	0.001	1.000
?	<55	33	24	9	?	?
肿瘤直径（cm）		?	?	?	?	?
?	≤2	16	12	4	0.515	0.773
?	2～5	53	39	14	?	?
?	≥5	11	7	4	?	?
肿瘤位置		?	?	?	?	?
?	胃窦	32	24	8	0.496	0.780
?	胃底	5	3	2	?	?
?	胃体	43	31	12	?	?
组织分型		?	?	?	?	?
?	高分化腺癌	6	1	5	22.425	<0.001
?	中分化腺癌	7	2	5	?	?
?	低分化腺癌	45	40	5	?	?
?	印戒细胞癌	22	15	7	?	?
肿瘤浸润深度		?	?	?	?	?
?	T1	8	5	3	25.171	<0.001
?	T2	35	23	12	?	?
?	T3	21	16	5	?	?
?	T4	16	14	2	?	?
淋巴结转移		?	?	?	?	?
?	无	15	1	14	21.002a	<0.001 a
?	微转移	23	19	4	?	?
?	有	42	38	4	?	?
远处转移		?	?	?	?	?
?	无	70	51	19	0.036	1.000
?	有	10	7	3	?	?

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