Expression of mismatch repair proteins hMLH1, hMSH2 and hMSH6 in sporadic colorectal cancer

doi:10.3877/cma.j.issn.1674-0793.2015.03.005

Abstract

Abstract:

Objective

To investigate the expression and clinical significance of mismatch repair proteins hMLH1, hMSH2 and hMSH6 in sporadic colorectal carcinoma (SCRC).

Methods

Two hundred and ninety patients with SCRC were studied, who underwent surgery in the Department of General Surgery, the Air Force General Hospital and the Department of Colorectal Surgery, Beijing Cancer Hospital from March 2008 to December 2012. Immunohistochemistry was used to detect hMLH1, hMSH2 and hMSH6 expression in tumor tissues. The relationship between expression and clinicopathological data was analyzed retrospectively.

Results

The absent expression rates of hMLH1, hMSH2 and hMSH6 only in tumor tissues were 13.4% (39/290), 12.1% (35/290) and 29.0% (84/290), respectively. The absent expression rates of hMLH1 and hMSH2, hMLH1 and hMSH6, hMSH2 and hMSH6, and hMLH1, hMSH2 and hMSH6 were 3.8% (11/290), 10.0% (29/290), 7.2% (21/290) and 3.4% (10/290), respectively. The absent expression rate of hMLH1 in highly differentiated adenocarcinoma tissues was obviously higher than that of moderately to poorly differentiated adenocarcinoma and mucous carcinomas tissues (P<0.01). In the tumor tissues less than 5 cm, the expression level of hMSH2 was obviously higher than that of more than 5 cm, so as hMSH6 (P<0.05). The expression rate of hMSH6 in female group was clearly higher than that of male group (P<0.01), and the expression rate of hMSH6 in high lymph node metastasis of tumor tissues was higher than that of low lymph node metastasis.

Conclusions

The absent expressions of hMLH1, hMSH2 and hMSH6 are common in SCRC, and the clinicopathological parameters of SCRC is obviously different from hereditary nonpolyposis colorectal cancer (HNPCC). Therefore, the biological effects, indicators for evaluation of degree of malignancy, individualized chemotherapy regimens and prognostic indicators may be different from those in HNPCC.

Key words: Mismatch repair protein, Sporadic colorectal cancer, Hereditary nonpolyposis colorectal cancer

Pengfei Yu, Xiang Fei, Xueming Wei, Ming Li, Jin Gu, Guoli Gu. Expression of mismatch repair proteins hMLH1, hMSH2 and hMSH6 in sporadic colorectal cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2015, 09(03): 193-197.

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References 20

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项目		例数	hMLH1				hMSH2				hMSH6
项目		例数	-	+	χ²值	P值	-	+	χ²值	P值	-	+	χ²值	P值
性别		?	?	?	0.07	0.795	?	?	1.12	0.289	?	?	20.07	<0.01
?	男	158	22	136	?	?	22	136	?	?	63	95	?	?
?	女	132	17	115	?	?	13	119	?	?	21	111	?	?
年龄(岁)		?	?	?	0.49	0.483	?	?	2.89	0.058	?	?	1.42	0.233
?	<60	119	14	105	?	?	19	100	?	?	39	80	?	?
?	≥60	171	25	146	?	?	16	155	?	?	45	126	?	?
肿瘤最大径(cm)		?	?	?	0.66	0.415	?	?	10.74	0.020	?	?	4.26	0.024
?	>5	95	15	80	?	?	20	75	?	?	35	60	?	?
?	≤5	195	24	171	?	?	15	180	?	?	49	146	?	?
部位		?	?	?	2.49	0.646	?	?	3.12	0.514	?	?	1.56	0.816
?	右半结肠	82	12	70	?	?	14	68	?	?	28	54	?	?
?	左半结肠	21	5	16	?	?	3	18	?	?	6	15	?	?
?	乙状结肠	77	9	68	?	?	7	70	?	?	20	57	?	?
?	直肠	110	13	97	?	?	11	99	?	?	30	80	?	?
形态		?	?	?	1.81	0.173	?	?	3.23	0.139	?	?	1.41	0.472
?	溃疡型	225	27	198	?	?	23	202	?	?	69	156	?	?
?	隆起型	65	12	53	?	?	12	53	?	?	15	50	?	?
浸润深度		?	?	?	0.10	0.953	?	?	5.08	0.227	?	?	2.46	0.367
?	肌层内	54	7	47	?	?	9	45	?	?	17	37	?	?
?	浆膜	203	27	176	?	?	19	184	?	?	54	149	?	?
?	浆膜外	33	5	28	?	?	7	26	?	?	13	20	?	?
分化程度		?	?	?	18.39	<0.01	?	?	2.72	0.322	?	?	0.89	0.570
?	高分化	20	9	11	?	?	4	16	?	?	4	16	?	?
?	中分化	183	20	163	?	?	18	165	?	?	55	128	?	?
?	低分化、黏液腺瘤	87	10	77	?	?	13	74	?	?	25	62	?	?
脉管内癌栓		?	?	?	0.13	0.654	?	?	0.71	0.398	?	?	4.83	0.028
?	有	66	8	58	?	?	6	60	?	?	12	54	?	?
?	无	224	31	193	?	?	29	195	?	?	72	152	?	?
淋巴结转移		?	?	?	1.61	0.389	?	?	2.46	0.615	?	?	16.70	0.003
?	0	124	20	104	?	?	11	113	?	?	44	80	?	?
?	1~3	95	12	83	?	?	15	80	?	?	33	62	?	?
?	≥4	71	7	64	?	?	9	62	?	?	7	64	?	?

项目		例数	hMLH1				hMSH2				hMSH6
项目		例数	-	+	χ²值	P值	-	+	χ²值	P值	-	+	χ²值	P值
性别		?	?	?	0.07	0.795	?	?	1.12	0.289	?	?	20.07	<0.01
?	男	158	22	136	?	?	22	136	?	?	63	95	?	?
?	女	132	17	115	?	?	13	119	?	?	21	111	?	?
年龄(岁)		?	?	?	0.49	0.483	?	?	2.89	0.058	?	?	1.42	0.233
?	<60	119	14	105	?	?	19	100	?	?	39	80	?	?
?	≥60	171	25	146	?	?	16	155	?	?	45	126	?	?
肿瘤最大径(cm)		?	?	?	0.66	0.415	?	?	10.74	0.020	?	?	4.26	0.024
?	>5	95	15	80	?	?	20	75	?	?	35	60	?	?
?	≤5	195	24	171	?	?	15	180	?	?	49	146	?	?
部位		?	?	?	2.49	0.646	?	?	3.12	0.514	?	?	1.56	0.816
?	右半结肠	82	12	70	?	?	14	68	?	?	28	54	?	?
?	左半结肠	21	5	16	?	?	3	18	?	?	6	15	?	?
?	乙状结肠	77	9	68	?	?	7	70	?	?	20	57	?	?
?	直肠	110	13	97	?	?	11	99	?	?	30	80	?	?
形态		?	?	?	1.81	0.173	?	?	3.23	0.139	?	?	1.41	0.472
?	溃疡型	225	27	198	?	?	23	202	?	?	69	156	?	?
?	隆起型	65	12	53	?	?	12	53	?	?	15	50	?	?
浸润深度		?	?	?	0.10	0.953	?	?	5.08	0.227	?	?	2.46	0.367
?	肌层内	54	7	47	?	?	9	45	?	?	17	37	?	?
?	浆膜	203	27	176	?	?	19	184	?	?	54	149	?	?
?	浆膜外	33	5	28	?	?	7	26	?	?	13	20	?	?
分化程度		?	?	?	18.39	<0.01	?	?	2.72	0.322	?	?	0.89	0.570
?	高分化	20	9	11	?	?	4	16	?	?	4	16	?	?
?	中分化	183	20	163	?	?	18	165	?	?	55	128	?	?
?	低分化、黏液腺瘤	87	10	77	?	?	13	74	?	?	25	62	?	?
脉管内癌栓		?	?	?	0.13	0.654	?	?	0.71	0.398	?	?	4.83	0.028
?	有	66	8	58	?	?	6	60	?	?	12	54	?	?
?	无	224	31	193	?	?	29	195	?	?	72	152	?	?
淋巴结转移		?	?	?	1.61	0.389	?	?	2.46	0.615	?	?	16.70	0.003
?	0	124	20	104	?	?	11	113	?	?	44	80	?	?
?	1~3	95	12	83	?	?	15	80	?	?	33	62	?	?
?	≥4	71	7	64	?	?	9	62	?	?	7	64	?	?

项目		hMLH1				hMSH2
项目		(+~++)	(-)	χ²值	P值	(+~++)	(-)	χ²值	P值
hMSH2		?	?	0.31	>0.05	?	?	?	?
?	(+~++)	227	28	?	?	-	-	-	-
?	(-)	24	11	?	?	-	-	?	?
hMSH6		?	?	31.15	<0.01	?	?	?	?
?	(+~++)	196	10	?	?	192	14	?	?
?	(-)	55	29	?	?	63	21	25.00	<0.01

项目		hMLH1				hMSH2
项目		(+~++)	(-)	χ²值	P值	(+~++)	(-)	χ²值	P值
hMSH2		?	?	0.31	>0.05	?	?	?	?
?	(+~++)	227	28	?	?	-	-	-	-
?	(-)	24	11	?	?	-	-	?	?
hMSH6		?	?	31.15	<0.01	?	?	?	?
?	(+~++)	196	10	?	?	192	14	?	?
?	(-)	55	29	?	?	63	21	25.00	<0.01

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