piRNAs derived from GAS5 activating tumor suppressor FOXO4 in breast cancer cell

doi:10.3877/cma.j.issn.1674-0793.2017.03.003

Abstract

Abstract:

Objective

To investigate the activation of FOXO4 by piRNAs derived from GAS5 in breast cancer cell.

Methods

A mixture of the five chemically synthesized single-stranded piRNAs, including pi-sno44/74/75/78/81, were transfected into MCF7 cells. Protein expression of FOXO4 and gene expression detected by microarray mRNA, and quantitative analysis of FOXO4 was performed from 29 clinical breast samples.

Results

FOXO4 expression was up-regulated in breast cancer cells, and the up-regulation was related to pi-sno44/74/75/81 (Corr=0.64, 0.91, 0.76, 0.93, P<0.001), but not to pi-sno78 (Corr=0.25, P=0.912).

Conclusion

piRNAs including pi-sno44/74/75/81 derived from GAS5 can mediate the induction of FOXO4, and may lead to the inhibition of tumor growth.

Key words: Breast neoplasms, Microchip analytical procedures, piRNA, FOXO4

Xinxin Chen, Ting Xia, Xiaowu Hu, Tengfei Cao, Lehong Zhang. piRNAs derived from GAS5 activating tumor suppressor FOXO4 in breast cancer cell[J]. Chinese Archives of General Surgery(Electronic Edition), 2017, 11(03): 154-158.

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Figures/Tables 5

References 16

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