Expression and clinical significance of combined detection of Cullin1 and MT4-MMP in breast cancer

doi:10.3877/cma.j.issn.1674-0793.2018.01.005

Abstract

Abstract:

Objective

To explore the expression and clinical significance of Cullin1 and MT4-MMP in different types of breast cancer tissue.

Methods

Eighty cases of breast cancer tissues and 80 cases of normal breast tissues adjacent to breast cancer were selected. Immunohistochemistry and Western blotting was used to detect the expression of Cullin1 and MT4-MMP in cancer tissue and normal breast tissue respectively between January 2015 and August 2015 in Xuzhou City Cancer Hospital, and to analyze the correlation between Cullin1 and MT4-MMP.

Results

The expressions of Cullin1 and MT4-MMP in breast cancer were significantly higher than in adjacent normal breast tissues (77.5% vs 28.8%, 67.5% vs 17.5%, χ²=38.174, 40.921, both P<0.01). In breast cancer tissues, the expression of Cullin1 and MT4-MMP protein was positively correlated (P<0.05). The expression of Cullin1 and MT4-MMP in breast cancer was statistically significant in molecular typing, axillary lymph node metastasis and TNM staging (P<0.05), but without significant difference in the age, tumor size, tumor location, recurrence and metastasis.

Conclusions

Cullin1 and MT4-MMP may be involved in the formation and development of breast cancer and seem to play a certain synergy role in this process. Higher expression level of Cullin1 and MT4-MMP in worse molecular typing and advanced breast cancer may become a new marker of malignant breast cancer.

Key words: Breast neoplasms, SKP Cullin F-box protein ligases, Matrix metalloproteinase 17

Yi Ren, Jing Li, Pengbo Zhang, Qing Zhong, Zeqiang Ren. Expression and clinical significance of combined detection of Cullin1 and MT4-MMP in breast cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2018, 12(01): 19-23.

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Figures/Tables 9

References 15

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组织来源	n	Cullin1的表达				阳性率（%）	χ²值	P值
组织来源	n	-	+	++	+++	阳性率（%）	χ²值	P值
乳腺癌	80	18	22	19	21	77.5	38.174	<0.01
癌旁正常乳腺	80	57	10	8	5	28.8	38.174	<0.01
组织来源	n	MT4-MMP的表达				阳性率（%）	χ²值	P值
组织来源	n	-	+	++	+++	阳性率（%）	χ²值	P值
乳腺癌	80	26	28	18	8	67.5	40.921	<0.01
癌旁正常乳腺	80	66	5	5	4	17.5	40.921	<0.01

组织来源	n	Cullin1的表达				阳性率（%）	χ²值	P值
组织来源	n	-	+	++	+++	阳性率（%）	χ²值	P值
乳腺癌	80	18	22	19	21	77.5	38.174	<0.01
癌旁正常乳腺	80	57	10	8	5	28.8	38.174	<0.01
组织来源	n	MT4-MMP的表达				阳性率（%）	χ²值	P值
组织来源	n	-	+	++	+++	阳性率（%）	χ²值	P值
乳腺癌	80	26	28	18	8	67.5	40.921	<0.01
癌旁正常乳腺	80	66	5	5	4	17.5	40.921	<0.01

项目		n	Cullin1的表达		阳性率（%）	χ²值	P值
项目		n	阳性	阴性	阳性率（%）	χ²值	P值
年龄（岁）
	<40	3	2	1	-	- ^a	>0.05
	≥40	77	60	17	77.9	- ^a	>0.05
肿瘤部位
	左侧	44	32	12	72.7	1.277	0.294
	右侧	36	30	6	83.3	1.277	0.294
肿瘤大小（cm）
	<2.0	35	28	7	80.0	0.223	0.789
	≥2.0	45	34	11	75.6	0.223	0.789
分子分型
	Luminal型	53	36	17	67.9	8.403	0.015
	Her-2阳性型	11	11	0	100.0
	三阴性型	16	15	1	93.8
淋巴结转移
	有	23	22	1	95.7	6.100	0.016
	无	57	40	17	70.2	6.100	0.016
TNM分期
	Ⅰ	45	34	11	75.6	9.869	0.007
	Ⅱ	26	20	6	76.9
	Ⅲ	9	8	1	88.9
复发转移
	有	6	5	1	83.3	- ^b	>0.05
	无	74	57	17	77.0	- ^b	>0.05

项目		n	Cullin1的表达		阳性率（%）	χ²值	P值
项目		n	阳性	阴性	阳性率（%）	χ²值	P值
年龄（岁）
	<40	3	2	1	-	- ^a	>0.05
	≥40	77	60	17	77.9	- ^a	>0.05
肿瘤部位
	左侧	44	32	12	72.7	1.277	0.294
	右侧	36	30	6	83.3	1.277	0.294
肿瘤大小（cm）
	<2.0	35	28	7	80.0	0.223	0.789
	≥2.0	45	34	11	75.6	0.223	0.789
分子分型
	Luminal型	53	36	17	67.9	8.403	0.015
	Her-2阳性型	11	11	0	100.0
	三阴性型	16	15	1	93.8
淋巴结转移
	有	23	22	1	95.7	6.100	0.016
	无	57	40	17	70.2	6.100	0.016
TNM分期
	Ⅰ	45	34	11	75.6	9.869	0.007
	Ⅱ	26	20	6	76.9
	Ⅲ	9	8	1	88.9
复发转移
	有	6	5	1	83.3	- ^b	>0.05
	无	74	57	17	77.0	- ^b	>0.05

项目		n	MT4-MMP的表达		阳性率（%）	χ²值	P值
项目		n	阳性	阴性	阳性率（%）	χ²值	P值
年龄（岁）
	<40	3	1	2	33.3	- ^a	0.245
	≥40	77	53	24	68.8	- ^a	0.245
肿瘤部位
	左侧	44	29	15	65.9	0.113	0.813
	右侧	36	25	11	69.4	0.113	0.813
肿瘤大小（cm）
	<2.0	35	24	11	68.6	0.033	0.857
	≥2.0	45	30	15	66.7	0.033	0.857
分子分型
	Luminal型	53	28	25	52.8	15.521	<0.001
	Her-2阳性型	11	11	0	100.0
	三阴性型	16	15	1	93.8
淋巴结转移
	有	23	22	1	95.7	11.662	<0.001
	无	57	32	25	56.1	11.662	<0.001
TNM分期
	Ⅰ	45	25	20	55.6	6.890	0.032
	Ⅱ	26	21	5	80.8
	III	9	8	1	88.9
复发转移
	有	6	4	2	66.7	- ^b	>0.05
	无	74	50	24	67.6	- ^b	>0.05

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