Expressions of lncRNA GHET1 and IGF2BP1 in colorectal cancer and their influence on prognosis

doi:10.3877/cma.j.issn.1674-0793.2021.02.001

Abstract

Abstract:

Objective

To investigate the expressions of long non-coding RNA gastric carcinoma highly expressed transcript 1 (lncRNA GHET1) and insulin-like growth factor mRNA-binding protein 1 (IGF2BP1) in colorectal cancer (CRC), and to explore their relationship with the prognosis of CRC.

Methods

From January 2014 to January 2015, tissue samples from 90 CRC patients with complete pathological and clinical data in South Hospital of the Sixth People’s Hospital Affiliated to Shanghai Jiaotong University were selected as the study group, and the adjacent tissue samples were taken as the control group. The expressions of GHET1 and IGF2BP1 were detected by real-time fluorescence quantitative PCR (qPCR) and immunohistochemistry. The relationship between the expressions of GHET1 and IGF2BP1 and clinicopathological features was analyzed. Kaplan-Meier method was used to analyze the relationship between the expressions of GHET1 and IGF2BP1 in CRC tissues and prognosis, while Cox regression analysis was used for the prognostic factors of CRC.

Results

The level of GHET1 mRNA and the positive expression rate of IGF2BP1 protein in the study group were significantly higher than those in the control group (P<0.05). The expressions of GHET1 mRNA and IGF2BP1 protein were correlated with tumor differentiation, depth of invasion, TNM stage and lymph node metastasis (P<0.05). Kaplan-Meier analysis showed that the survival rate of patients with high expression of GHET1 mRNA and IGF2BP1 protein positive was lower than patients with low expression of GHET1 and IGF2BP1 negative (32.80% vs 75.00%, χ²=17.775; 33.33% vs 80.00%, χ²=13.615; both P<0.05). Multivariate Cox analysis showed that high expression of GHET1 mRNA and positive expression of IGF2BP1 protein were risk factors for the prognosis of CRC (P<0.05).

Conclusion

There are high expressions of GHET1 mRNA and IGF2BP1 protein in CRC tissues, which are closely related to the poor prognosis of patients.

Key words: Gastric carcinoma highly expressed transcript 1, Insulin-like growth factorⅡ mRNA-binding protein 1, Colorectal neoplasms, Expression, Prognosis

Ping Zhang, Zhanhai Tan, Jianrong Chen, Jifa Zhang, Lianming Zhou, Yuanzhou Shan, Qin Zhao. Expressions of lncRNA GHET1 and IGF2BP1 in colorectal cancer and their influence on prognosis[J]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(02): 81-85.

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References 14

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miRNA	正向引物5’→3’	反向引物5’→3’
GHET1	GAACAAAGCAGGTAAACATTGG	GCAAAGGCAGAGTGAAAGGT
GAPDH	GAGTCAACGGATTTGGTCGT	GACAAGCTTCCCGTTCTCAG

miRNA	正向引物5’→3’	反向引物5’→3’
GHET1	GAACAAAGCAGGTAAACATTGG	GCAAAGGCAGAGTGAAAGGT
GAPDH	GAGTCAACGGATTTGGTCGT	GACAAGCTTCCCGTTCTCAG

临床病理参数		例数	GHET1 mRNA表达情况				IGF2BP1蛋白表达情况
临床病理参数		例数	高表达(58例)	低表达(32例)	χ²值	P值	阳性(60例)	阴性(30例)	χ²值	P值
年龄(岁)					2.102	0.147			0.022	0.881
	<60	43	31	12			29	14
	≥60	47	27	20			31	16
性别					0.005	0.944			0.023	0.880
	男	53	34	19			35	18
	女	37	24	13			25	12
肿瘤位置					0.357	0.550			0.356	0.551
	结肠	44	27	17			28	16
	直肠	46	31	15			32	14
肿瘤大小(cm)					1.832	0.176			0.201	0.654
	<5	42	24	18			29	13
	≥5	48	34	14			31	17
分化程度					5.989	0.014			5.830	0.016
	低	52	39	13			40	12
	中、高	38	19	19			20	18
浸润深度					5.757	0.016			5.081	0.024
	T1+T2	28	13	15			14	14
	T3+T4	62	45	17			46	16
TNM分期					5.436	0.020			4.498	0.034
	Ⅰ~Ⅱ期	37	25	22			20	17
	Ⅲ~Ⅳ期	53	33	10			40	13
淋巴结转移					5.463	0.019			5.208	0.022
	是	36	18	18			19	17
	否	54	40	14			41	13

临床病理参数		例数	GHET1 mRNA表达情况				IGF2BP1蛋白表达情况
临床病理参数		例数	高表达(58例)	低表达(32例)	χ²值	P值	阳性(60例)	阴性(30例)	χ²值	P值
年龄(岁)					2.102	0.147			0.022	0.881
	<60	43	31	12			29	14
	≥60	47	27	20			31	16
性别					0.005	0.944			0.023	0.880
	男	53	34	19			35	18
	女	37	24	13			25	12
肿瘤位置					0.357	0.550			0.356	0.551
	结肠	44	27	17			28	16
	直肠	46	31	15			32	14
肿瘤大小(cm)					1.832	0.176			0.201	0.654
	<5	42	24	18			29	13
	≥5	48	34	14			31	17
分化程度					5.989	0.014			5.830	0.016
	低	52	39	13			40	12
	中、高	38	19	19			20	18
浸润深度					5.757	0.016			5.081	0.024
	T1+T2	28	13	15			14	14
	T3+T4	62	45	17			46	16
TNM分期					5.436	0.020			4.498	0.034
	Ⅰ~Ⅱ期	37	25	22			20	17
	Ⅲ~Ⅳ期	53	33	10			40	13
淋巴结转移					5.463	0.019			5.208	0.022
	是	36	18	18			19	17
	否	54	40	14			41	13

变量	单因素分析			多因素分析
变量	HR	95% CI	P值	HR	95% CI	P值
淋巴结转移(是vs否)	1.357	1.073~1.717	0.011	-	-	-
TNM分期(Ⅰ~Ⅱ期vs Ⅲ~Ⅳ期)	1.260	1.015~1.563	0.036	-	-	-
浸润深度(T1+T2 vs T3+T4)	1.369	1.061~1.766	0.016	-	-	-
分化程度(低vs中、高)	1.891	1.253~2.854	0.002	-	-	-
GHET1(高表达vs低表达)	1.443	1.118~1.861	0.005	1.479	1.169~1.871	0.001
IGF2BP1(阳性vs阴性)	1.534	1.121~2.099	0.007	1.368	1.103~1.697	0.004

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