BANCR promoting lymphangiogenesis by regulating VEGF-C/VEGFR-3 pathway in pancreatic cancer

doi:10.3877/cma.j.issn.1674-0793.2021.04.006

Abstract

Abstract:

Objective

To investigate the expression of BANCR in pancreatic carcinoma and its mechanism on lymphangiogenesis.

Methods

The expression of BANCR in pancreatic cancer cells (SW1990, PANC-1) and human pancreatic ductal epithelial cell (HPDC) was detected by qPCR. siRNA was used to interfere with the expression of BANCR in pancreatic cancer cells. The transfected pancreatic cancer cells and human dermal lymphatic endothelial cells (HDLEC) were co-cultured in 3D, and micro-lymphatic vessel density (MLVD) was calculated. qPCR was used to detect the relative expression of VEGF-C and VEGFR-3 mRNA in pancreatic cancer cells of BANCR-siRNA group and NC group.

Results

Compared with 1.02±0.222 of HPDC, the expression of BANCR in PANC-1 and SW1990 cells was significantly higher (10.03±3.356, 10.37±1.459, respectively) (P<0.001). The pancreatic cancer cell MLVD in the BANCR-siRNA group was significantly lower than that in the NC group (SW1990: 3.87±1.767 vs 18.00±6.130, P<0.001; PANC-1: 5.27±2.631 vs 16.80±3.764, P<0.001). And the transcriptional level of VEGF-C and VEGFR-3 in the BANCR-siRNA group was significantly lower than that in the NC group (VEGF-C: 1.35±0.926 vs 6.97±3.677, P=0.011; VEGFR-3: 0.98±0.635 vs 2.88±1.422, P=0.026).

Conclusion

BANCR is up-regulated in pancreatic carcinoma cells and may play an important role in lymphangiogenesis and lymph node metastasis in pancreatic carcinoma by regulating VEGF-C/VEGFR-3 pathway, which is expected to provide a new target for clinical diagnosis, treatment and prognosis of pancreatic carcinoma.

Key words: Pancreatic neoplasms, Long chain non-coding RNA, Lymphangiogenesis

Shaolong Hao, Wei Han, Yu Ji, Hao Sun, Haowei Shi, Jihong Ma. BANCR promoting lymphangiogenesis by regulating VEGF-C/VEGFR-3 pathway in pancreatic cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2021, 15(04): 269-272.

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References 16

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基因名称	上游引物	下游引物
BANCR	5’-GAGCCTTGCCAGTTCCATTT-3’	5’-TGCAGAGGTGAGATTCAGGT-3’
GAPDH	5’-TCAAGAAGGTGGTGAAGCAGG-3’	5’-TCAAAGGTGGAGGAGTGGGT-3’
BANCR siRNA	5’-GGUGTGGCGUCTUTUGCUUTT-3’	5’-AAGCAAAAGACGCCACACCTT-3’
VEGF-C	5’-CTACAGATGTGGGGGTTGCT-3’	5’-GATTGGCAAAACTGATTGTGAC-3’
VEGFR-3	5’-CTCTGACCTAGTGGAGATCCTG-3’	5’-CTTCGGTGATATGTAGAGCTGTG-3’

基因名称	上游引物	下游引物
BANCR	5’-GAGCCTTGCCAGTTCCATTT-3’	5’-TGCAGAGGTGAGATTCAGGT-3’
GAPDH	5’-TCAAGAAGGTGGTGAAGCAGG-3’	5’-TCAAAGGTGGAGGAGTGGGT-3’
BANCR siRNA	5’-GGUGTGGCGUCTUTUGCUUTT-3’	5’-AAGCAAAAGACGCCACACCTT-3’
VEGF-C	5’-CTACAGATGTGGGGGTTGCT-3’	5’-GATTGGCAAAACTGATTGTGAC-3’
VEGFR-3	5’-CTCTGACCTAGTGGAGATCCTG-3’	5’-CTTCGGTGATATGTAGAGCTGTG-3’

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