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Chinese Archives of General Surgery(Electronic Edition) ›› 2021, Vol. 15 ›› Issue (04): 288-292. doi: 10.3877/cma.j.issn.1674-0793.2021.04.010

• Original Article • Previous Articles     Next Articles

miR-877-3p regulating the proliferation, migration and invasion of breast cancer cells via HER-2/PI3K/AKT signaling pathway

Xin Li1, Mi Tian2, Lili He1,()   

  1. 1. Department of Thyroid and Breast Surgery, Jingmen No.2 People’s Hospital, Jingmen 448000, China
    2. Forensic Identification Center of Jingmen Public Security Bureau, Jingmen 448000, China
  • Received:2020-11-15 Online:2021-08-03 Published:2021-08-18
  • Contact: Lili He

Abstract:

Objective

To explore the molecular mechanism of miR-877-3p targeting HER-2 and regulating its downstream PI3K/AKT signaling pathway to mediate the proliferation, migration, invasion and healing functions of breast cancer.

Methods

The expression of miR-877-3p and HER-2 was evaluated in normal breast epithelial cells and breast cancer cells using qPCR and Western blotting assay. Furthermore, the proliferation, migration, invasion and healing functions of miR-877-3p were analyzed in breast cancer cells by CCK-8, Transwell and healing experiments. Additionally, the targeting relationship betweenmiR-877-3p and HER-2 was detected by luciferase reporter gene experiment. The expression levels of miR-877-3p targeting HER-2 and its downstream PI3K/AKT signaling pathway proteins were evaluated by Western blotting assay.

Results

miR-877-3p expression was negatively correlated with HER-2 in breast cancer cells. The proliferation, migration, invasion and healing functions were supressed by miR-877-3p in breast cancer cell. HER-2 was a direct target of miR-887-3p. miR-877-3p targeted HER-2 to regulate PI3K/AKT signaling pathway.

Conclusion

miR-877-3p targets HER-2/PI3K/AKT signaling pathway to mediate the proliferation, migration and invasion of breast cancer.

Key words: Breast neoplasms, miR-877-3p, HER-2/PI3K/AKT

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