Abstract:

The importance and diversification of gut microbiota in human health and disease are increasingly recognized. The difficulties in inferring causal relationships from metagenomic microbiome sequencing studies and differences between mouse and human species have promoted the development of complex in vitro models of human gut microbiome interactions. This article will review the latest progress in co-culture of microorganisms with intestinal and colonic epithelium, and compare the rapidly developing organoid model with other standard model, describe how the specific individual process of microbial epithelial interaction can be reproduced in vitro and discuss the advantages of co-cultivation models of human gut microbiota and organoid model in clinical application prospects, so as to explore the new direction of individualized treatment.

Key words: Organoids, Gastrointestinal microbiome, Coculture techniques, Personalized treatment