Signal transduction regulation of nucleoprotein receptor in hepatocellular carcinoma

doi:10.3877/cma.j.issn.1674-0793.2007.03.004

Abstract

Abstract:

Objective

To investigate the correlation of PPARγ and PTEN mRNA expressions of hepatocellular carcinoma（HCC）tissue samples and the effect of both genes on human HCC cell line BEL-7402 cell growth.

Methods

RT-PCR analysis was performed to detect the mRNA expression levels of PPARγ and PTEN in 21 HCC samples. The expression of PTEN protein were assessed by Western blot analysis. The cell growth status of BEL-7402 cells was evaluated by MTT assay.

Results

The mRNA expression of PTEN and PPARγ decreased in HCC tissues， compared with adjacent nontumor liver tissues， and both the mRNA expressions indicated positive correlation (r ＝0.774， P<0.01).Rosiglitazone， as a synthetic ligands of PPARγ， inhibited BEL-7402 cell growth and increased PTEN protein expression by PPARγ pathway.

Conclusion

PTEN and PPARγ gene expression present positive correlation in HCC tissues. Rosiglitazone activate PPARγ pathway to increase PTEN expression， which may be the molecular mechanism of rosiglitazone-inhibited BEL-7402 cell growth.

Key words: Peroxisome proliferator-activated receptor γ, PTEN gene, Hepatocellular carcinoma

Liangqi CAO, Xi-lin CHEN, Qian WANG. Signal transduction regulation of nucleoprotein receptor in hepatocellular carcinoma[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(03): 136-138.

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