The role of cellular immunity in the xenotransplantation of hepatocytes in donor and recipient combination of tilapia to rat

doi:10.3877/cma.j.issn.1674-0793.2007.03.006

Abstract

Abstract:

Objective

To investigate the rejection mechanisms of cellular immune in xenogeneic hepatocytes transplantation between tilapia and rat.

Methods

The outbred SD rats were divided randomly into two groups， the control group and transplantation group. And the tilapia(donor) hepatocytes(2×10⁷) were transplanted into the spleen of the rat(recipient) in the transplantation group ，normal sodium was injected into the spleen of the rat (recipient) in the control group. The histological characteristics of transplanted tilapia hepatocytes were observed by HE staining. The CD₄/CD₈ lymphocytes around the grafts were detected with immunohistochemistrical method.

Results

Grafted hepatocytes disappeared quickly after transplantation. Only a few living hepatocytes could be found in spleens after 8 hours. The CD₄/CD₈ lymphocytes were congregated around the grafts at 4 hours post-graft.

Conclusion

The cellular immune responses participate in the rejection of xenogeneic hepatocytes transplantation between tilapia and rat.

Key words: Immunity cellular, Vertebrates, Graft Rejection, Hepatocytes

Zhu-qing ZHOU, Zhong-xin ZHAO. The role of cellular immunity in the xenotransplantation of hepatocytes in donor and recipient combination of tilapia to rat[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(03): 143-145.

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