Abstract:

Background and Aims

Previously we have documented that lung injury after trauma-hemorrhagic shock（T/HS）is significant 3 hours after resuscitation and that females are more resistant toT/HS-induced lung injury than males.However，it is not known if the lung injury and its gender dimorphrism still exsit 5 days after resuscitation.Therefore，We aim to determine the lung injury after T/HS and to compare its gender difference 5 days after resuscitation.

Methods

Male and female Sprague-Dawley rats were subjected to a midline laparotomy（trauma）and either hemorrhagic shock or sham shock.At the end of the shock period，the animals were resuscitated with Ringer lactate 3 times of their shed blood.Five days postresuscitation，the animals were sacrificed and lung permeability was measured.

Results

Lung permeability and pulmonary leukosequestration in T/HS animals were increased significantly 5 days.Females subjected to T/HS had increased pulmonary permeability，increased BALF protein/plasma protein ratio and increased pulmonary myeloperoxidase levels than males.Males gained while females lost body weight during study period.

Conclusion

Lung injury still can be seen 5 days aftar resuscitation.The injury is more severe in female than in male.

Key words: Trauma/hemorrhagic shock, Gender, Rat, Lung injury