Effect of T cell vaccine in inducing rat tolerance of allogeneic skin transplantation

doi:10.3877/cma.j.issn.1674-0793.2007.04.006

Abstract

Abstract:

Objective

To investigate the preparation and the effect of T cell vaccine（TCV）in inducing immune tolerance of allogeneic in skin transplantation.

Methods

TCV made from Wistar rat recipients with the specific antigen of SD donor rats was used to immunize the rat recipients by peritoneal injection.Before and after the fifth day of the every times vaccination，one-way mixed lymphocyte reaction（MLR）were performed with effective cells of peripheral T cells of recipient rats and stimulator cells of peripheral T cells of SD rats，stimulator cells were attenuated by Mitomycin-C preliminary.To observed the effect of immune tolerance induction by TCV in rats，The skin grafts from SD rats were transplanted to Wistar rats.The grafts reactions and survival time were observed.Skin grafts histopathological examination were used as a reference to evaluate the reject reaction.

Results

The immunological responses were suppressed significantly after TCV vaccination（P﹤0.05）.The surviving time and acute rejection of transplanted skins were better in TCV group（P﹤0.05）.

Conclusion

In rat model，immunological tolerance can be successfully induced by TCV.TCV vaccination can increase the antirejection and surviving time of skin allografts.

Key words: Cell vaccine, Skin transplantation, Immune tolerance

Jinqing HUANG, De CHEN, Shi-kun QIAN, Xing-yuan JIAO, Hang LIN, Hai HU. Effect of T cell vaccine in inducing rat tolerance of allogeneic skin transplantation[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(04): 208-211.

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Figures/Tables 2

References 12

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组别	A ₅₇₀值
组别	接种前	第1次接种后第5天	第2次接种后第5天	第3次接种后第5天
TCV1组	0.39±0.023	0.21±0.022	0.14±0.021	0.04±0.031（88.8%）
TCV2组	0.39±0.031	0.36±0.021	0.30±0.023	0.23±0.021（41%）
对照组	0.38±0.051	0.39±0.032	0.40±0.041	0.39±0.031

组别	A ₅₇₀值
组别	接种前	第1次接种后第5天	第2次接种后第5天	第3次接种后第5天
TCV1组	0.39±0.023	0.21±0.022	0.14±0.021	0.04±0.031（88.8%）
TCV2组	0.39±0.031	0.36±0.021	0.30±0.023	0.23±0.021（41%）
对照组	0.38±0.051	0.39±0.032	0.40±0.041	0.39±0.031

组别	例数（n）	供体	受体	存活时间（d）
TCV1组	15	SD	WISTAR	27.2±2.3
TCV2组	15	SD	WISTAR	18.4±1.8
对照组	15	SD	WISTAR	11.5±1.2

组别	例数（n）	供体	受体	存活时间（d）
TCV1组	15	SD	WISTAR	27.2±2.3
TCV2组	15	SD	WISTAR	18.4±1.8
对照组	15	SD	WISTAR	11.5±1.2

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