Relationship between hepatocellular apoptosis and tumor necrosis factor-alpha in rats during hepatic ischemia-reperfusion.

doi:10.3877/cma.j.issn.1674-0793.2008.01.006

Abstract

Abstract:

Objective

To investigate the relationship between hepatocellular apoptosis and tumor necrosis factor-alpha(TNF-α)in rats during hepatic ischemia-reperfusion.

Methods

With a rat model of acute hepatic ischemia-reperfusion,72 healthy male Wistar rats were randomly divided into 3 groups:normal group, sham-operated group and ischemia-reperfusion group. The concentrations of serum TNF-α was measured by radioimmunity technique and the hepatocellular apoptosis was detected by TdT-mediated dUTP-biotin nick end labeling(TUNEL) and electron microscope.

Results

The concentrations of serum TNF-α and hepatocellular apoptotic index(HAI)in ischemia-reperfusion group were higher than that in normal and sham-operated groups.Both TNF-α and HAI reached peak at 6 hour after reperfusion. There was a positive correlation between TNF-α and HAI.

Conclusion

There is an extraordinary up-regulation of the expression of TNF-α after hepatic ischemia-reperfusion and perhaps TNF-α is an inducible factor of hepatocellular apoptosis.

Key words: Liver, Ischemia-reperfusion injury, TNF-α, Apoptosis

Kai SUN, Zhi-su LIU. Relationship between hepatocellular apoptosis and tumor necrosis factor-alpha in rats during hepatic ischemia-reperfusion.[J]. Chinese Archives of General Surgery(Electronic Edition), 2008, 02(01): 13-16.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2008.01.006

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2008/V02/I01/13

Figures/Tables 4

References 7

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组别	1 h	3 h	6 h	24 h
N	0.58±0.11
S	0.61±0.10	0.69±0.14	0.67±0.12	0.70±0.17
IR	1.17±0.64*^#	1.37±0.72*^#	2.06±0.76*^#	1.51±0.69*^#

组别	1 h	3 h	6 h	24 h
N	0.58±0.11
S	0.61±0.10	0.69±0.14	0.67±0.12	0.70±0.17
IR	1.17±0.64*^#	1.37±0.72*^#	2.06±0.76*^#	1.51±0.69*^#

组别	1 h	3 h	6 h	24 h
N	3.14±0.83
S	3.51±0.95	3.36±0.76	3.69±0.72	3.90±0.93
IR	27.69±3.16*#	31.69±3.44*#	47.80±3.47*#	35.95±3.11*#

组别	1 h	3 h	6 h	24 h
N	3.14±0.83
S	3.51±0.95	3.36±0.76	3.69±0.72	3.90±0.93
IR	27.69±3.16*#	31.69±3.44*#	47.80±3.47*#	35.95±3.11*#

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