Photodynamic therapy of hematoporphyrin derivative on human cholangiocarcinoma cell

doi:10.3877/cma.j.issn.1674-0793.2008.01.007

Abstract

Abstract:

Objective

To investigate the killing effect of photodynamic therapy(PDT) on human cholangiocarcinoma cell in vitro,and the dosage-effect relation of photo sensitizer and laser light in photodynamic therapy.

Methods

Human cholangiocarcinoma cells were cultured with serial concentrations of hematoporphyrin derivatives (HPD) followed by irradiation of different dosage of laser light,then MTT colorimetric assay was applied to measure the relative inhibitory rate of PDT for the cells.Use SPSS software to find out the best parameter for killing effect of PDT. Observe the different killing effect of three groups of efficiency-time set-up.

Results

Significant difference in the relative inhibitory between the PDT group and control group was observed (P＜0.01).The relative inhibitory rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of laser light.Relative inhibitory rate of the lower concentration and high laser light dose group will be the same as that of higher concentration HPD and lower laser light dose group.

Conclusion

PDT has significant killing effect on human cholangioncarcinoma cells, and its relative inhibitory rate appears to be correlated with the dose of sensitizer and laser light irritation. Low concentration and high laser light dose set-up is the effective and safe module of PDT.Higher laser light dose and shorter time irradiation will be the best pattern of PDT.

Key words: Photodynamic therapy, Hematoporphyrin derivative, Human Cholangiocarcinoma Cell

Zi-long WEN, Ping XUE, Hai-wu LU, Yi-ze HU. Photodynamic therapy of hematoporphyrin derivative on human cholangiocarcinoma cell[J]. Chinese Archives of General Surgery(Electronic Edition), 2008, 02(01): 17-20.

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Figures/Tables 2

References 7

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HPD(mg/L)	PDT(J/cm²)
HPD(mg/L)	0	5	10	15
0	0.7280±0.0199	0.7298±0.0429	0.6257±0.0190	0.6538±0.0314
4	0.7349±0.0919	0.3381±0.0187^#	0.2770±0.0217^#	0.0577±0.0080^#
6	0.7424±0.1905	0.1354±0.0216*	0.0732±0.0080*	0.0445±0.0090*
8	0.7032±0.0840	0.0929±0.0100*	0.0608±0.0090*	0.0413±0.0100*
10	0.7099±0.0980	0.0655±0.0060*	0.0429±0.0080*	0.0413±0.0110*
12	0.6917±0.0660	0.0696±0.0100*	0.0362±0.0050*	0.0169±0.0060*
14	0.7245±0.0960	0.0188±0.0030*	0.0229±0.0071*	0.0293±0.0101*

HPD(mg/L)	PDT(J/cm²)
HPD(mg/L)	0	5	10	15
0	0.7280±0.0199	0.7298±0.0429	0.6257±0.0190	0.6538±0.0314
4	0.7349±0.0919	0.3381±0.0187^#	0.2770±0.0217^#	0.0577±0.0080^#
6	0.7424±0.1905	0.1354±0.0216*	0.0732±0.0080*	0.0445±0.0090*
8	0.7032±0.0840	0.0929±0.0100*	0.0608±0.0090*	0.0413±0.0100*
10	0.7099±0.0980	0.0655±0.0060*	0.0429±0.0080*	0.0413±0.0110*
12	0.6917±0.0660	0.0696±0.0100*	0.0362±0.0050*	0.0169±0.0060*
14	0.7245±0.0960	0.0188±0.0030*	0.0229±0.0071*	0.0293±0.0101*

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