Manumycin-induced apoptosis via reactive oxygen species in KAT-4 anaplastic thyroid cancer cells

doi:10.3877/cma.j.issn.1674-0793.2008.04.008

Abstract

Abstract:

Objective

To investigate the effects of manumycin on KAT-4 anaplastic thyroid cancer cell line, and to explore the mechanism.

Methods

Human anaplastic thyroid cancer cells KAT-4 were used.The cytotoxicity was analyzed by MTT assay.Apoptosis and cellular nitric oxide were detected by flow cytometry using annexin v and NO sensor dye.Superoxide anion was measured with a fluorescent plate reader by DHE.GSH was assayed by fluorescent monochlorobimane.

Results

Manumycin resulted in viability decrease in a dose-dependent manner.Manumycin induced apoptosis and ROS generation including nitric oxide and superoxide anions.Manumycin reduced intracellular glutathione.Manumycin-induced apoptosis correlated with increase in ROS.Quenching of ROS with N-acetyl-L-cysteine protected leukemia cells from the cytotoxicity of manumycin and prevented apoptosis induction by manumycin.

Conclusion

Cellular ROS generation plays an important role in the cytotoxic effect of manumycin. Manumycin induces apoptosis through ROS generation in KAT-4 cells.

Key words: Anaplastic thyroid cancer, Manumycin, Apoptosis, Reactive oxygen species

Jin-gao LI, Miaorong SHE, Hu-hui HUANG, Xia WAN. Manumycin-induced apoptosis via reactive oxygen species in KAT-4 anaplastic thyroid cancer cells[J]. Chinese Archives of General Surgery(Electronic Edition), 2008, 02(04): 282-286.

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Figures/Tables 5

References 12

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