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Chinese Archives of General Surgery(Electronic Edition) ›› 2009, Vol. 03 ›› Issue (02): 18-20. doi: 10.3877/cma.j.issn.1674-0793.2009.02.006

• Original Articles • Previous Articles     Next Articles

Effect of hypoxia-inducible factor 1α regulated expression on hepatoma cell proliferation and apoptosis in vitro

Bai-lin WANG1,(), Shu-ping ZHAI1, Zhen-yu XIAO1, Xian-ming ZHAO1, Wen-tao ZHAO1   

  1. 1.the Second Department of Surgery, the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
  • Received:2008-12-02 Online:2009-04-01 Published:2024-11-30
  • Contact: Bai-lin WANG

Abstract:

Objective

To observe the effects of hypoxia-inducible factor 1α (HIF-1α)regulated expression on hepatoma cell proliferation and apoptosis in vitro.

Methods

The Tet-on gene regulated HIF-1α was transfected to HepG2 cell lines and the apoptosis rate,expressions of proliferative protein CyclinD1,CyclinA gene and apoptosis protein Survivin,Bcl-2 gene were detected,HepG2Tet-on cell apoptosis were induced by different concentrations of doxycycline.

Results

After being incubated under different concentrations of doxycycline for 48 h, HIF-1α gene could increase HepG2Tet-on cell proliferation and inhibit it's apoptosis, The apoptosis rates were 56.4%±7.8%, 42.7%±4.9%, 31.5%±6.1%, 19.7%±4.5% respectively when the concentraions doxycycine were 0,0.1,1,5 mg/L(P<0.01).The mRNA expression intensity of HIF-1α,cyclinD1,cyclinA,Survivin,Bcl-2 increased along with the concentration increased of doxycycline (P<0.01).

Conclusions

HIF-1α gene can increase the proliferation of hepatoma cells and inhibit their apoptosis in vitro in an expression level dependent manner.CyclinD1,CyclinA,Survivin,Bcl-2 expression are involved in the possible mechanism.

Key words: Hepatocellularcarcinoma, Cellproliferation, Apoptosis, Hypoxia-induciblefactor1α, Tet-on geneexpressionsystem

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