Abstract:
Objective
To observe the effects of hypoxia-inducible factor 1α (HIF-1α)regulated expression on hepatoma cell proliferation and apoptosis in vitro.
Methods
The Tet-on gene regulated HIF-1α was transfected to HepG2 cell lines and the apoptosis rate,expressions of proliferative protein CyclinD1,CyclinA gene and apoptosis protein Survivin,Bcl-2 gene were detected,HepG2Tet-on cell apoptosis were induced by different concentrations of doxycycline.
Results
After being incubated under different concentrations of doxycycline for 48 h, HIF-1α gene could increase HepG2Tet-on cell proliferation and inhibit it's apoptosis, The apoptosis rates were 56.4%±7.8%, 42.7%±4.9%, 31.5%±6.1%, 19.7%±4.5% respectively when the concentraions doxycycine were 0,0.1,1,5 mg/L(P＜0.01).The mRNA expression intensity of HIF-1α,cyclinD1,cyclinA,Survivin,Bcl-2 increased along with the concentration increased of doxycycline (P＜0.01).
Conclusions
HIF-1α gene can increase the proliferation of hepatoma cells and inhibit their apoptosis in vitro in an expression level dependent manner.CyclinD1,CyclinA,Survivin,Bcl-2 expression are involved in the possible mechanism.
Key words:
Hepatocellularcarcinoma,
Cellproliferation,
Apoptosis,
Hypoxia-induciblefactor1α,
Tet-on geneexpressionsystem
Bai-lin WANG, Shu-ping ZHAI, Zhen-yu XIAO, Xian-ming ZHAO, Wen-tao ZHAO. Effect of hypoxia-inducible factor 1α regulated expression on hepatoma cell proliferation and apoptosis in vitro[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(02): 18-20.