Abstract:
Objective
CD44 is an adhesion molecule of cell surface, which is closely related with metastasis and prognosis of breast cancer,but the relationship between CD44 and drug resistance of breast cancer chemotherapy is not clear so far. We mainly study the relationship between CD44 and drug resistance of breast cancer chemotherapy.
Methods
The expression of CD44 was detected by SP immunohistochemical methods,the different expression of CD44 was investigated between drug-sensitive groups and drug-resistant groups of neoadjuvant chemotherapy,before and after chemotherapy.The different expressions of CD44 on the level of mRNA and protein in MCF-7 cell line and drug-resistant cell lines MCF-7/ADR was tested by reverse transcription polymerase chain reaction (RT-PCR) and flow cytometry.
Results
Expression of CD44 before chemotherapy in drug-resistant groups was much higher than drug-sensitive ones(χ2=4.22，P <0.05). This was also found after chemotherapy(χ2=9.954，P<0.01).The expression of CD44 after chemotherapy was higher than that before chem otherapy (t＝3.43，P<0.01).Expression of mRNA and protein of CD44 in MCF-7/ADR cell lines was higher than drug-sensitive cell lines (MCF-7).
Conclusion
CD44 may be conferred as a predictive index of breast cancer chemotherapy drug resistance,and chemotherapy may enrich CD44 positive breast cancer cell.
Key words:
CD44,
Breastcancer,
Neoadjuvantchemotherapy,
Drugresistance
Herui YAO, Zhi-hua LI, Yong-hui LUO, Feng-xi SU. Research of the relationship between CD44 and resistance of breast cancer chemotherapy[J]. Chinese Archives of General Surgery(Electronic Edition), 2009, 03(04): 280-284.