Establishment of a new portacaval transposition model

doi:10.3877/cma.j.issn.1674-0793.2011.02.003

Abstract

Abstract:

Objective

To explore the feasibility of a new portacaval transposition model establish.

Methods

The allograft rat model of portacaval transposition was established by using vascular cuff way. At the end of the installation of a cuff of left renal vein, the left renal vein and the portal vein stump by cuff connected, affixed above the left renal vein ligation and cut the inferior vena cava, the inferior vena cava by allogeneic cuff and superior mesenteric vessels and venous connection. Another 10 rats served as controls after resection of the left kidney. Postoperative body weight in rats, biochemical parameters, MRI and pathological changes of the liver were detected.

Results

The survival rate of experimental group were 97.50% (39/40) after 2 weeks. Compared with control group, albumin, alanine aminotransferase, alkaline phosphatase and total bilirubin, weight and other indicators of experimental group showed no significant difference after 2 months. Pathological examination were normal.

Conclusion

Using allograft vascular cuff to establish rat model of portacaval transposition is feasible.

Key words: Portacaval transposition, Allograft vascular, Animal model, Rat

Fan-lin ZENG, Yi JIANG, Xiao-jin ZHANG, Jun-zhen WU. Establishment of a new portacaval transposition model[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(02): 96-99.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2011.02.003

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Figures/Tables 5

References 7

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项目	对照组	实验组	t值	P值
白蛋白(g/L)	30.800±2.781	30.300±2.163	0.449	0.659
ALT(U/L)	68.600±30.783	61.800±12.804	0.645	0.527
ALP(U/L)	189.900±89.923	190.500±71.683	0.016	0.987
TBIL(μmol/L)	1.390±0.981	1.330±0.726	0.155	0.878
体重(g)	398.600±10.114	395.400±12.522	0.445	0.668

项目	对照组	实验组	t值	P值
白蛋白(g/L)	30.800±2.781	30.300±2.163	0.449	0.659
ALT(U/L)	68.600±30.783	61.800±12.804	0.645	0.527
ALP(U/L)	189.900±89.923	190.500±71.683	0.016	0.987
TBIL(μmol/L)	1.390±0.981	1.330±0.726	0.155	0.878
体重(g)	398.600±10.114	395.400±12.522	0.445	0.668

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