Astilbin protecting hepatic ischemia-reperfusion injury in rats through the pathway of autophagy

doi:10.3877/cma.j.issn.1674-0793.2018.02.003

Abstract

Abstract:

Objective

To explore the protective effect of Astilbin in hepatic ischemia-reperfusion injury (HIRI).

Methods

Fifty-four SD rats were divided into Sham group (control group), HIRI group (ischemia-reperfusion group) and Astilbin group (40 mg·kg^-1·d^-1 for continuous 7 days) with 18 in each group, to establish the model of rat HIRI. After the liver was reperfused with blood (in 4, 8, 16 h), the specimens of blood and liver tissues were collected. Serum alanine aminotransferase (ALT) and aspertate aminotransferase (AST) were detected by automatic biochemical analyzer. The changes of liver cell microstructure were observed under the optical microscope. The expression of autophagy related protein LC3 and Beclin-1 in liver tissue were detected by Western blotting analysis. The number of autophagosome was observed using electron microscope.

Results

Compared with the HIRI group at each detection time point, serum ALT and AST levels of the sham group and Astilbin group were significantly lower (P<0.05), with less liver cell swelling, inflammatory cell infiltration, and the structural damage of the sinks. LC3- / LC3-ratio and the gray value of Beclin-1 protein in the Astilbin group were lower than that of group HIRI at 4, 8, 16 h after operation (P<0.05). The number of autophagosomes in liver tissue of Astilbin group was also less than the HIRI group (3.68±0.42 vs 7.12±0.60, t=36.382, P<0.01).

Conclusion

Astilbin pretreatment can reduce HIRI and its mechanism may be associated with inhibiting the autophagy.

Key words: Astilbin, Ischemia-reperfusion, Liver, Autophagy

Feng Xue, Yubin Xu, Xunan Mao, Jie Zhang, Lide Tao, Guangcai Xu, Peijian Zhang. Astilbin protecting hepatic ischemia-reperfusion injury in rats through the pathway of autophagy[J]. Chinese Archives of General Surgery(Electronic Edition), 2018, 12(02): 81-84.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2018.02.003

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2018/V12/I02/81

Figures/Tables 7

References 10

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组别	n	4 h	t值	P值	8 h	t值	P值	16 h	t值	P值
Sham组	6	57.11±6.32	18.36	P<0.05	54.93±5.59	17.26	P<0.05	55.86±7.25	8.21	P<0.05
HIRI组	6	640.12±50.02	-	-	963.33±40.45	-	-	253.31±16.84	-	-
落新妇苷组	6	203.36±23.25	9.37	P<0.05	368.36±22.35	13.32	P<0.05	96.64±8.72	9.52	P<0.05

组别	n	4 h	t值	P值	8 h	t值	P值	16 h	t值	P值
Sham组	6	57.11±6.32	18.36	P<0.05	54.93±5.59	17.26	P<0.05	55.86±7.25	8.21	P<0.05
HIRI组	6	640.12±50.02	-	-	963.33±40.45	-	-	253.31±16.84	-	-
落新妇苷组	6	203.36±23.25	9.37	P<0.05	368.36±22.35	13.32	P<0.05	96.64±8.72	9.52	P<0.05

组别	n	4 h	t值	P值	8 h	t值	P值	16 h	t值	P值
Sham组	6	165.66±11.16	17.48	P<0.05	158.43±11.23	21.35	P<0.05	160.78±16.32	10.25	P<0.05
HIRI组	6	896.39±40.91	-	-	1603.85±60.32	-	-	489.53±22.31	-	-
落新妇苷组	6	312.49±15.68	13.21	P<0.05	496.39±28.72	15.39	P<0.05	210.27±20.74	16.36	P<0.05

组别	n	4 h	t值	P值	8 h	t值	P值	16 h	t值	P值
Sham组	6	165.66±11.16	17.48	P<0.05	158.43±11.23	21.35	P<0.05	160.78±16.32	10.25	P<0.05
HIRI组	6	896.39±40.91	-	-	1603.85±60.32	-	-	489.53±22.31	-	-
落新妇苷组	6	312.49±15.68	13.21	P<0.05	496.39±28.72	15.39	P<0.05	210.27±20.74	16.36	P<0.05

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