MicroRNA-21 promoting the invasion and metastasis of cholangiocarcinoma through epithelial-mesenchymal transition

doi:10.3877/cma.j.issn.1674-0793.2018.02.004

Abstract

Abstract:

Objective

To investigate the effect of microRNA-21 (miR-21) on the invasionand metastasis of cholangiocarcinoma RBE cells, as well as its underlying mechanisms.

Methods

Cholangiocarcinoma RBE cells were transfected miR-21 or miR-21 inhibitor (miR-21i) mediated in lentivirus. Reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of miR21. Transwell chambers and wound-healing assay were applied to measure the invasive and metastatic effects of RBE cells. Western blotting analysis was used to assess the expression of PTEN protein and epithelial-mesenchymal transition (EMT) related proteins, including E-cadherin, N-cadherin, and Vimentin.

Results

The over-expression of miR-21 promoted invasion and metastasis of cholangiocarcinoma RBE cells, miR-21 inhibitor suppressed these effects. Furthermore, it has been found that the over-expression of miR-21 up-regulated PTEN and E-cadherin proteins, and down-regulated N-cadherin and Vimentin proteins. However, these data were not observed in the control group.

Conclusions

miR-21 acts as an oncogene, promotes invasion and metastasis of cholangiocarcinoma cells through PTEN and EMT. These findings provide new scientific basis.

Key words: Bile duct neoplasms, MicroRNAs, Genes, suppressor, Epithelial-mesenchymal transition, PTEN

Liangqi Cao, Yue Li, Nan He, Zhangjun Wei, Dawei Zhang, Xuewei Yang. MicroRNA-21 promoting the invasion and metastasis of cholangiocarcinoma through epithelial-mesenchymal transition[J]. Chinese Archives of General Surgery(Electronic Edition), 2018, 12(02): 85-89.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2018.02.004

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2018/V12/I02/85

Figures/Tables 4

References 12

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