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Chinese Archives of General Surgery(Electronic Edition) ›› 2018, Vol. 12 ›› Issue (05): 301-305. doi: 10.3877/cma.j.issn.1674-0793.2018.05.004

Special Issue:

• Original Article • Previous Articles     Next Articles

Circulating tumor DNA in monitoring the dynamic changes of tumor burden during treatment of metastatic breast cancer

Shunying Li1, Yichao Fang1, Hongna Lai1, Yudong Li1, Yujie Liu1,()   

  1. 1. Department of Breast Cancer, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Received:2018-08-03 Online:2018-10-01 Published:2018-10-01
  • Contact: Yujie Liu
  • About author:
    Corresponding author: Liu Yujie, Email:

Abstract:

Objective

To explore the utility of circulating tumor DNA (ctDNA) in evaluating tumor burden and tracking the changes of tumor clones in metastatic breast cancer patients.

Methods

High-throughput sequencing and target region capture was applied in our study to analyze ctDNA sequence in different time points from metastatic breast cancer patients and was compared with the imaging indexes.

Results

The study included thirteen blood samples collected from 5 advanced breast cancer patients at different time points. Next generation sequencing detected ctDNA in 12 out of the 13 samples. Bioinformatics revealed the dynamic changes of ctDNA subclones in metastatic breast cancer patients during treatment.

Conclusion

ctDNA assay is applicable in advanced breast cancer patients and ctDNA can effectively reflect the changes of tumor burden.

Key words: Breast neoplasms, Neoplasm metastasis, Neoplastic cells, circulating, Driver mutation

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