Abstract:
Objective To evaluate the effects of congestion postconditioning on acute intestinal venous congestion reperfusion injury in rats.
Methods A total of twenty-four male Sprague-Dawley (SD) rats were randomly assigned to three groups: the acute intestinal venous congestion reperfusion group (CR group, n=8), the congestion postconditioning group (CPO group, n=8) and the sham group (Sham group, n=8). In CR group, portal vein was clamped for 45 min, then reperfusion for 60 min to induce intestinal congestion reperfusion injury. In CPO group, the same procedure plus postconditioning (portal vein was open for 30 s, then clamped for 30 s with three cycles of this procedure) initiated immediately at the onset of portal vein reperfusion. In Sham group, all the procedures were the same as CR group except for clamping portal vein. After reperfusion ileum samples were collected for Chiu’s scores and wet-to-dry weight. The blood of portal vein was collected for detection of TNF-α and endotoxin in serum.
Results (1) The serum concentrations of TNF-α in the portal vein of rats in the Sham, CR, and CPO groups were (31.37±2.39), (114.75±7.46), (89.37±5.83) μg/L, and the endotoxin concentrations were (0.64±0.16), (5.04±0.28) and (4.00±0.31) EU/ml, respectively. The serum concentrations of TNF-α and endotoxin were significantly higher in the CR group than in the Sham group. The CPO group was significantly lower than the CR group. The differences were statistically significant (F=404.84, 680.85, both P<0.01). (2) The pathological lesions of the small bowel in the CR group had Qiu score of 4.01±0.35, which was significantly higher than 0.43±0.21 of the Sham group and 3.58±0.34 of CPO group (Z=6.18, 4.25, both P<0.01). (3) In the CR group, the wet to dry ratio of the small intestine was 5.29±0.36, which was significantly higher than 3.34±0.23 of the Sham group and 4.26±0.26 of CPO group (F=2.03, 0.44, P=0.003, 0.012).
Conclusion Congestion postconditioning has protective effects on congestion and reperfusion injury of small intestinal mucosa, and its protective mechanism may be to delay and reduce the release of inflammatory mediators.
Key words:
Reperfusion injury,
Intestine small,
Models animal,
Congestion postconditioning
Wancheng Niu, Shiping Wang, Xi Li, Feng Zhou, Deben Li, Zhaohui Zhang. Study on the protective effect of congestion postconditioning on acute intestinal venous congestionreperfusion injury in rats[J]. Chinese Archives of General Surgery(Electronic Edition), 2018, 12(05): 306-309.