Mechanism of ulinastatin post-treatment on learning and memory function after partial hepatectomyin aged rats

doi:10.3877/cma.j.issn.1674-0793.2018.05.006

Abstract

Abstract:

Objective

To investigate the effects of ulinastatin post-treatment on the learning and memory after partial hepatectomy in aged rats.

Methods

Thirty-six healthy C57BL/6J male mice (weighed 27-36 g) were randomly divided into three groups: control group (group C), partial hepatectomy group (group PH), and ulinastatin post-treatment group (group UTI), with 12 in each group. After being performed partial hepatectomy, 50 000 U/kg ulinastatin was injected intraperitoneally for 7 consecutive days in UTI group, while the equal volume of normal saline was given in group C and group PH. 24 hours after the last injection, the rats underwent Morris water maze and open-filed test. The animals were sacrificed and their hippocampi were immediately removed for determination of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1 (IL-1) using ELISA and expression of Amloyid-β protein, protein tau, protein tau phospho-396 in the hippicampus of the cells using Western blotting.

Results

Compared with group C and group UTI, the escape latency on the third and fourth day and swimming distance in group PH were significantly prolonged (F=11.783, 72.141, 70.807, all P<0.001), the exploring time and the crossing times were shortened (F=143.244, 32.428, both P<0.001), esidence time in the central district and span numbers, the erect frequency were shortened in the open field (F=76.232, 105.990, 17.477, all P<0.001) , and the expression of TNF-α, IL-6 and IL-1 was up-regulated (F=395.545, 1 124.503, 474.526, all P<0.001), the levels of hippocampal tau protein, tau phospho-396 and Amloyid-β protein were increased in group PH (F=285.764, 307.440, 557.347, all P<0.001).

Conclusion

Ulinastatin can improve the cognitive function after partial hepatectomy in old mice and inhibition of inflammatory responses, the expession of hippocampal tau protein, tau phospho-396 and Amloyid-β proteinin hippocampi is involved in the mechanism.

Key words: Hepatectomy, Rats, Learning, Memory, Ulinastatin

Yu Hong, Jiayi Liu, Shouping Wang, Lei Zhang. Mechanism of ulinastatin post-treatment on learning and memory function after partial hepatectomyin aged rats[J]. Chinese Archives of General Surgery(Electronic Edition), 2018, 12(05): 310-313.

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References 16

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组别	n	逃避潜伏期（s）				探索时间（s）	游泳距离（cm）	穿梭原平台次数（次）
组别	n	第1天	第2天	第3天	第4天	探索时间（s）	游泳距离（cm）	穿梭原平台次数（次）
C组	12	49.6±4.7	35.2±4.1	20.9±2.5	13.5±1.1	30.9±2.4	324.5±68.3	12.8±3.1
PH组	12	50.2±5.3	34.3±5.7	29.5±3.4^a	19.9±1.8^a	16.4±1.2^a	543.6±71.9^a	5.1±1.9^a
UTI组	12	48.8±4.2	36.4±4.2	21.3±2.8^b	14.3±1.3^b	31.1±2.6^b	313.2±61.8^b	11.9±3.4^b
F值	?	0.042	0.024	11.783	72.141	143.244	70.807	32.428
P值	?	0.963	0.976	<0.001	<0.001	<0.001	<0.001	<0.001

组别	n	逃避潜伏期（s）				探索时间（s）	游泳距离（cm）	穿梭原平台次数（次）
组别	n	第1天	第2天	第3天	第4天	探索时间（s）	游泳距离（cm）	穿梭原平台次数（次）
C组	12	49.6±4.7	35.2±4.1	20.9±2.5	13.5±1.1	30.9±2.4	324.5±68.3	12.8±3.1
PH组	12	50.2±5.3	34.3±5.7	29.5±3.4^a	19.9±1.8^a	16.4±1.2^a	543.6±71.9^a	5.1±1.9^a
UTI组	12	48.8±4.2	36.4±4.2	21.3±2.8^b	14.3±1.3^b	31.1±2.6^b	313.2±61.8^b	11.9±3.4^b
F值	?	0.042	0.024	11.783	72.141	143.244	70.807	32.428
P值	?	0.963	0.976	<0.001	<0.001	<0.001	<0.001	<0.001

组别	n	中央区探索时间（s）	跨格次数（次）	直立次数（次）
C组	12	64.5±15.7	53.4±16.8	21.3±7.4
PH组	12	32.3±6.8^a	30.1±5.5^a	15.7±4.9^a
UTI组	12	66.4±12.5^b	52.7±13.8^b	20.4±6.6^b
F值	?	76.232	105.990	17.477
P值	?	<0.001	<0.001	<0.001

组别	n	中央区探索时间（s）	跨格次数（次）	直立次数（次）
C组	12	64.5±15.7	53.4±16.8	21.3±7.4
PH组	12	32.3±6.8^a	30.1±5.5^a	15.7±4.9^a
UTI组	12	66.4±12.5^b	52.7±13.8^b	20.4±6.6^b
F值	?	76.232	105.990	17.477
P值	?	<0.001	<0.001	<0.001

组别	n	TNF-α	IL-6	IL-1
C组	12	3.55±0.15	1.86±0.11	3.27±0.22
PH组	12	7.03±0.81^a	4.86±0.37^a	9.75±0.61^a
UTI组	12	3.62±0.19^b	1.72±0.13^b	3.49±0.26^b
F值	?	395.545	1 124.503	474.526
P值	?	<0.001	<0.001	<0.001

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