Granulocyte macrophage colony stimulating factor enhancing drug resistance of gastric cancer cells by inhibiting apoptosis

doi:10.3877/cma.j.issn.1674-0793.2020.01.004

Abstract

Abstract:

Objective

By detecting the effects of granulocyte macrophage colony stimulating factor (GM-CSF) on the apoptosis of gastric cancer cells induced by fluorouracil (5-FU), the mechanism of GM-CSFinvolved in drug resistance of gastric cancer cells was preliminarily explored.

Methods

The gastric cancer SGC7901 cells were cultured in vitro, and the semi-lethal dose of 5-FU on gastric cancer cells was detected by MTT assay. GM-CSF was added to 5-FU-treated gastric cancer cells. The effects of cell proliferation and clonal proliferation were detected by MTT and plate cloning assay. Apoptosis was detected by Annexin V-FITC/PI flow cytometry. Western blotting was used to detect the expression differences of apoptosis-related proteins Bax and Bcl-2.

Results

The semi-lethal dose of 5-FU to gastric cancer cells was (16±0.4) mg/L.5-FU could inhibit the activity and proliferation of gastric cancer cells, which could be effectively weakened by GM-CSF (P<0.05). 5-FU induced apoptosis of gastric cancer cells significantly, Bax/Bcl-2 ratio was significantly increased, while GM-CSF could reverse these trend (P<0.05).

Conclusion

GM-CSF can promote the proliferation of gastric cancer cells and effectively inhibit the apoptosis of gastric cancer cells induced by 5-FU, and plays an important role in enhancing the chemoresistance of gastric cancer cells.

Key words: Granulocyte-macrophage colony-stimulating factor, Stomach neoplasms, Apoptosis, Drug resistance, neoplasm, Fluorouracil

Shilin Chang, Jinpeng He, Yaqiong Chen, Hui Cai. Granulocyte macrophage colony stimulating factor enhancing drug resistance of gastric cancer cells by inhibiting apoptosis[J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(01): 10-13.

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