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Chinese Archives of General Surgery(Electronic Edition) ›› 2020, Vol. 14 ›› Issue (02): 84-88. doi: 10.3877/cma.j.issn.1674-0793.2020.02.002

Special Issue:

• Original Article • Previous Articles     Next Articles

Role of intracellular imatinib concentration in drug resistance of gastrointestinal stromal tumor

Youchun Sheng1, Qiang Zhang1, Ming Chen1, Kangjing Xu1, Hao Xu1,()   

  1. 1. Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2019-05-09 Online:2020-04-01 Published:2020-04-01
  • Contact: Hao Xu
  • About author:
    Corresponding author: Xu Hao, Email:

Abstract:

Objective

To investigate the relationship between intracellular imatinib concentration and drug resistance of gastrointestinal stromal tumor (GIST).

Methods

Eight advanced GIST patients with imatinib-resistant and -susceptible were randomly selected from the First Affiliated Hospital of Nanjing Medical University from December 2014 to April 2016. Concentrations of imatinib in cells, tissues and plasma were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The morphological characteristics of cells were observed by hematoxylin-eosin staining and immunohistochemistry.

Results

The imatinib concentration in tissues of imatinib-resistant patients was lower than that of imatinib-susceptible patients (P<0.05). Compared with parental sensitive cell lines, imatinib concentration of imatinib-resistant cell lines decreased significantly and cell morphology was also changed.

Conclusion

Low intracellular drug concentration may be an important reason of imatinib resistance, and intracellular imatinib concentration may be a key point in the treatment of GIST.

Key words: Gastrointestinal neoplasms, Drug resistance, neoplasm, Imatinib, Inhibitory concentration 50

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