Relationship among the expression of vascular endothelial growth factor C, platelet-derived growth factor-AA and -BB, lymphangiogenesis and lymph node metastasis in rectal cancer

doi:10.3877/cma.j.issn.1674-0793.2020.06.007

Abstract

Abstract:

Objective

To investigate the expression of vascular endothelial growth factor C (VEGF-C), platelet-derived growth factor (PDGF)-AA and PDGF-BB in rectal cancer tissues and their relationship with lymphangiogenesis and lymph node metastasis.

Methods

The expression of VEGF-C, PDGF-AA and PDGF-BB in 60 cases of rectal cancer (the rectal cancer group) and 30 cases of normal tissues (the control group) were detected by immunohistochemical staining (SP method). The microlymphatics density (LMVD) in rectal cancer was measured by D2-40, and the correlated analysis was conducted.

Results

The positive expression rates of VEGF-C, PDGF-AA and PDGF-BB in the rectal cancer tissues were significantly higher than those in control group (VEGF-C: 63.3% vs 3.3%, P<0.05; PDGF-AA: 40.0% vs 13.3%, P<0.05; PDGF-BB: 55.0% vs 6.7%, P<0.05). The positive rates of VEGF-C and PDGF-BB in lymph node positive group were significantly higher than those in lymph node negative group (VEGF-C: 86.4% vs 50.0%, P<0.01; PDGF-BB: 77.3% vs 42.1%, P<0.05), but there was no evidence show that PDGF-AA was correlated with lymph node metastasis (54.5% vs 31.6%, P>0.05). LMVD in the lymph node positive group was significantly higher than that in the lymph node negative group [(13.11±2.01) /mm² vs (8.13±2.99) /mm², P<0.05], LMVD in VEGF-C, PDGF-AAand PDGF-BB positive expression group was significantly higher than that of negative expression group[VEGF-C: (12.06±2.20)/mm² vs (6.32±2.41)/mm², P<0.05; PDGF-AA: (11.89±2.46)/mm² vs(8.67±3.67)/mm², P<0.05; PDGF-BB: (12.19±2.22)/mm² vs (7.33±3.02)/mm², P<0.05 ]. The expression of VEGF-C, PDGF-AA and PDGF-BB in rectal cancer was significantly correlated (VEGF-C vs PDGF-AA: r_s=0.366, P<0.05; VEGF-C vs PDGF-BB: r_s=0.650, P<0.05; PDGF-AA vs PDGF-BB: r_s=0.320, P<0.05).

Conclusions

Lymphangiogenesis is one of the important reasons for lymph node metastasis of rectal cancer. VEGF-C, PDGF-AA and PDGF-BB all can promote lymph node metastasis by participating in lymphangiogenesis of rectal cancer, and they may have a certain synergistic effect. Combined medication for VEGF-C, PDGF-AA and PDGF-BB may be more conducive to the prevention and treatment of lymph node metastasis of rectal cancer.

Key words: Rectal neoplasms, Lymphangiogenesis, Vascular endothelial growth factor-C, Platelet-derived growth factor-AA, Platelet-derived growth factor-BB

Kaihuang Lin, Weirong Chen, Xigui Chen. Relationship among the expression of vascular endothelial growth factor C, platelet-derived growth factor-AA and -BB, lymphangiogenesis and lymph node metastasis in rectal cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2020, 14(06): 429-433.

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Figures/Tables 6

References 13

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淋巴结转移	n	VEGF-C		PDGF-AA		PDGF-BB
淋巴结转移	n	阳性	阴性	阳性	阴性	阳性	阴性
阳性组	22	19(86.4)	3(13.6)	12(54.5)	10(45.5)	17(77.3)	5(22.7)
阴性组	38	19(50.0)	19(50.0)	12(31.6)	26(68.4)	16(42.1)	22(57.9)
χ²值		6.731		3.165		5.197
P值		<0.01		>0.05		<0.05

淋巴结转移	n	VEGF-C		PDGF-AA		PDGF-BB
淋巴结转移	n	阳性	阴性	阳性	阴性	阳性	阴性
阳性组	22	19(86.4)	3(13.6)	12(54.5)	10(45.5)	17(77.3)	5(22.7)
阴性组	38	19(50.0)	19(50.0)	12(31.6)	26(68.4)	16(42.1)	22(57.9)
χ²值		6.731		3.165		5.197
P值		<0.01		>0.05		<0.05

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