Expression of MMP9 and TIMP1 gene in primary liver cancer

doi:10.3877/cma.j.issn.1674-0793.2007.01.010

Abstract

Abstract:

Objective

To investigate the expression of MMP9 and TIMP1 gene and its role of the invasion and metastasis in primary liver cancer （PLC）.

Methods

MMP9 and TIMP1 mRNA expression in 34 PLCs and 12 normal liver specimens was detected by RT-PCR.

Results

Compared with the control group，MMP9 mRNA expression increased，but TIMP1 mRNA expression decreased in PLCs，especially in those with metastasis （P＜0.05）.There was a negative correlation between TIMP1 and MMP9 mRNA expression （P ＜0.01）.

Conclusion

MMP9 mRNA up-expression and TIMP1 mRNA down-expression might be related to the invasion and metastasis in PLC.

Key words: MMP9, TIMP1, primary liver cancer, metastasis

Mingyi LI, Jinke YAO, Yongguang YANG. Expression of MMP9 and TIMP1 gene in primary liver cancer[J]. Chinese Archives of General Surgery(Electronic Edition), 2007, 01(01): 33-35.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2007.01.010

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2007/V01/I01/33

Figures/Tables 2

正常	TIMP1/GAPDH		MMP9/GAPDH
正常	n	$\overset{ˉ}{x}$ ±s	n	$\overset{ˉ}{x}$ ±s
原发性肝癌	12	1.6048±0.1191	12	0.3721±0.0229
转移癌组织	19	0.4542±0.1015	19	1.1447±0.1258
转移癌旁组织	19	1.4668±0.2305	19	0.3634±0.0258
未转移癌组织	15	0.6324±0.0999	15	1.0121±0.1274
未转移癌旁组织	15	1.5220±0.2119	15	0.3559±0.0255

正常	TIMP1/GAPDH		MMP9/GAPDH
正常	n	$\overset{ˉ}{x}$ ±s	n	$\overset{ˉ}{x}$ ±s
原发性肝癌	12	1.6048±0.1191	12	0.3721±0.0229
转移癌组织	19	0.4542±0.1015	19	1.1447±0.1258
转移癌旁组织	19	1.4668±0.2305	19	0.3634±0.0258
未转移癌组织	15	0.6324±0.0999	15	1.0121±0.1274
未转移癌旁组织	15	1.5220±0.2119	15	0.3559±0.0255

	TIMP1/GAPDH		MMP9/GAPDH
	n	$\overset{ˉ}{x}$ ±s	n	$\overset{ˉ}{x}$ ±s
分化
分化好	20	0.7017±0.3190	20	1.0631±0.1546
分化差	14	0.7233±0.3408	14	1.1192±0.1174
肿瘤大小
＜5cm	14	0.7677±0.3448	14	1.0952±0.1035
≥5cm	20	0.6706±0.3098	20	1.0797±0.1651

	TIMP1/GAPDH		MMP9/GAPDH
	n	$\overset{ˉ}{x}$ ±s	n	$\overset{ˉ}{x}$ ±s
分化
分化好	20	0.7017±0.3190	20	1.0631±0.1546
分化差	14	0.7233±0.3408	14	1.1192±0.1174
肿瘤大小
＜5cm	14	0.7677±0.3448	14	1.0952±0.1035
≥5cm	20	0.6706±0.3098	20	1.0797±0.1651

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