Abstract:

Objective

To explore the repairing material and operational skill for bile duct defect.

Methods

40 local healthy hybrid goats were divided randomly into 4 group. In group A， B， C， Excision of a segmental duct (2cm， 3cm， 4cm at length respectively) was performed to establish bile duct injury models.Autologous vein segment with a stent was used to repair the common bile defects. Finally the vein graft was covered with vascularized great omentum. Bladders in all animals were excised. Cholecystectomy and a circular mobilization of the common bile duct were conducted in group D. Animals' postoperative performance status， blood biochemical examination on operative day and postoperative months 1， 3 and 6 were studied. Six months after the operation the animals were killed and histopathological change in the vein grafts was observed.

Results

No notable changes in blood biochemistry postoperatively. The typical columnar epithelium of the common bile duct was seen in the vain graft. There were much difference of postoperative performances of vein grafts performances after operation among A and B， C groups.

Conclusion

Transplantation of autologous great saphenous vein graft and stent covered by vascularized great omentum to repair common bile duct(CBD)injuries may be feasible and alternative in the goats. Further experimental studies should be carried out to validate these findings， which will help to clinical application.

Key words: Autologous great saphenous vein graft, Stent, Common bile duct, Repair