Effect of Aitongke on proliferation, apoptosis of human gastric cancer cells SGC-7901 and its mechanisms

doi:10.3877/cma.j.issn.1674-0793.2008.04.006

Abstract

Abstract:

Objective

To investigate the anti-cancer mechanisms of aitongke,its impact on proliferation,apoptosis and its underlying mechanisms in human gastric cancer cells SGC-7901.

Methods

SGC-7901 cells were treated by aitongke of different concentrations,the proliferating rate,the apoptosis rate,cell cycle and retinoblastoma(Rb)gene mRNA level change of SGC-7901 cells were evaluated by CCK method,flow cytometry analysis and RT-PCR method respectively.

Results

Aitongke (2～50mg/L)could effectivelyinhibited SGC-7901 cellproliferationandinducedcellapoptosis,bothwithatime-dosedependentmanner.Cellsaccumulated in thegap 1 (G1)phases,whilereduced inthegap2and mitosis (G2/M)phasesofthecellcycle,and theapoptosispeak significantly occurred.Aitongke could upregulate the expression of Rb gene,and with a dose dependent manner.

Conclusion

Aitongke has a potential role on anti-gastric cancer by inhibiting proliferation of SGC-7901 cell,as well as inducing cell apoptosis.And the apoptosis-inducing mechanism is mediated through the G1 blocking of cell cycle in SGC-7901 cells involved with the up-regulating expression of Rb gene.

Key words: Aitongke, Gastric cancer, Apoptosis

Huan-qing XIAO, Jie YANG, Zheng SUN, Shu-hua LI. Effect of Aitongke on proliferation, apoptosis of human gastric cancer cells SGC-7901 and its mechanisms[J]. Chinese Archives of General Surgery(Electronic Edition), 2008, 02(04): 275-278.

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URL: https://zhptwkxwx.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0793.2008.04.006

https://zhptwkxwx.cma-cmc.com.cn/EN/Y2008/V02/I04/275

Figures/Tables 4

References 8

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分组	剂量(mg/L)	G1期	S期	G2/M期
对照组	0	43.4±1.16	37.2±1.30	20.4±1.97
癌痛克组	2	52.9±0.89^*	36.0±2.87	11.1±1.87
	10	54.4±1.23^*	33.4±4.39	12.2±6.13
	50	65.5±2.47^#	26.3±8.42	8.2±5.87

分组	剂量(mg/L)	G1期	S期	G2/M期
对照组	0	43.4±1.16	37.2±1.30	20.4±1.97
癌痛克组	2	52.9±0.89^*	36.0±2.87	11.1±1.87
	10	54.4±1.23^*	33.4±4.39	12.2±6.13
	50	65.5±2.47^#	26.3±8.42	8.2±5.87

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