Abstract:
Objective
To study the modulation of heat shock protein 70 (HSP70)expression by ethyl pyruvate (EP)in the hepatocyte of septic mice.
Methods
Cecal ligation-perforation (CLP)models were made in mice as septic model.Total of 60 mice were divided into three groups:sham group,CLP+REPS group and CLP+RLS group.Ringer's ethyl pyruvate solution(REPS)and Ringer's lactic solution(RLS)were used in two groups to resuscitate the septic mice respectively.Malonaldehyde(MDA)in hepatic tissue and hepatocyte HSP70 expression was detected in different groups.
Results
Malondialdehyde level in septic groups was higher than in sham group (P<0.01).EP promoted the anti-oxidative ability of hepatic tissue in septic mice.Malondialdehyde level was lower in REPS group than in RLS group[(48.18±5.98)μmol·g-1protein vs(78.34±11.16) μmol·g-1protein, P<0.01]. The expression of hepatocytic HSP70 in REPS group was higher than in RLS group [(28.76±5.69)vs (20.04±4.93),P<0.05].The MDA level was negative correlated with the expression of HSP70 (r＝-0.733，P<0.01).
Conclusion
Ethyl pyruvate, as its anti-oxidative ability, can increase the expression of HSP70 of hepatocyte in septic mice.
Key words:
Sepsis,
Ethyl pyruvate,
Heat shock protein 70
Min-ying CHEN, Juan CHEN, Yang-bin OU, Xiang-dong GUAN. Modulation of hepatocyte heat shock protein 70 expression by ethyl pyruvate in septic mice[J]. Chinese Archives of General Surgery(Electronic Edition), 2008, 02(05): 363-366.