Protective effects of exogenous phosphocreatine on myocardial damage induced by hemorrhagic shock and its mechanisms

doi:10.3877/cma.j.issn.1674-0793.2011.03.007

Abstract

Abstract:

Objective

To observe protective effects of exogenous phosphocreatine on myocardial damage induced by hemorrhagic shock and its mechanisms.

Methods

Twenty healthy white rabbits were randomly divided into Control group (Group N) and Exogenous Phosphocreatine group (Group CP). Acute hemorrhagic shock animal model was established according to Wigger’s method. The changes of mean arterial pressure (MAP), serum creatine phosphokinase(CK) and cardiac Troponin I (cTn-I), myocardial mitochondrial ATPase, apoptosis and myocardial morphological structure were observed.

Results

There were excessive myocardial injuries in Group N, while in Group CP the myocardial myofilament disarray and the pathological finding were not as severe as in Group N. Compared with the group N, the level of CK and cTnI significantly decreased during reperfusion, the activity of mitochondrial Na⁺-K⁺-ATPase and Ca²⁺-ATPase significantly increase and the total number of staining myocytes of CP group were lower(P<0.05).

Conclusion

CP can protect markedly the myocardial damage induced by hemorrhagic shock.

Key words: Exogenous phosphocreatine, Hemorrhagic shock, Energy metabolism, Mitochondrial injury, Apoptosis

qin ZHOU, Qing-chun LIANG, Peng YANG, Xiao-yun DUAN, Wen-qi HUANG. Protective effects of exogenous phosphocreatine on myocardial damage induced by hemorrhagic shock and its mechanisms[J]. Chinese Archives of General Surgery(Electronic Edition), 2011, 05(03): 206-210.

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Figures/Tables 5

References 9

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组别	休克前	休克	期	复苏期
组别	休克前	0 min	60 min	15 min	30 min	60 min	90 min	120 min
N组	89.0±8.8	41.4±1.6	39.6±3.8	57.8±11.8	66.4±11.6	65.5±9.7	64.1±7.8	68.3±8.5
CP组	96.9±9.1	43.4±3.5	40.9±3.6	79.9±9.0^△	80.2±9.4^*	80.3±9.1^*	80.7±6.7^*	83.0±8.7^*

组别	休克前	休克	期	复苏期
组别	休克前	0 min	60 min	15 min	30 min	60 min	90 min	120 min
N组	89.0±8.8	41.4±1.6	39.6±3.8	57.8±11.8	66.4±11.6	65.5±9.7	64.1±7.8	68.3±8.5
CP组	96.9±9.1	43.4±3.5	40.9±3.6	79.9±9.0^△	80.2±9.4^*	80.3±9.1^*	80.7±6.7^*	83.0±8.7^*

组别		休克前（T₀）	休克60 min（T₁）	复苏期
组别		休克前（T₀）	休克60 min（T₁）	30 min（T₂）	60 min（T₃）	120 min（T₄）
CK		?	?	?	?	?
?	N组	505.0±118.02	640.6±130.52^*	1274.4±210.54^★	1551.0±248.95^#	1907.2±128.68^#
?	CP组	533.6±93.64	662.4±104.93^*	951.8±187.54^★△	1124.0±214.86^★△	1438.8±370.01^★△
cTnI		?	?	?	?	?
?	N组	0.0085±0.0012	0.0298±0.0054^*	0.0782±0.0247^#	0.1195±0.0332^#	0.1278±0.0278^#
?	CP组	0.0090±0.0014	0.0278±0.0051^*	0.0406±0.0083^△★	0.0590±0.0142^☆★	0.0638±0.0146^☆#

组别		休克前（T₀）	休克60 min（T₁）	复苏期
组别		休克前（T₀）	休克60 min（T₁）	30 min（T₂）	60 min（T₃）	120 min（T₄）
CK		?	?	?	?	?
?	N组	505.0±118.02	640.6±130.52^*	1274.4±210.54^★	1551.0±248.95^#	1907.2±128.68^#
?	CP组	533.6±93.64	662.4±104.93^*	951.8±187.54^★△	1124.0±214.86^★△	1438.8±370.01^★△
cTnI		?	?	?	?	?
?	N组	0.0085±0.0012	0.0298±0.0054^*	0.0782±0.0247^#	0.1195±0.0332^#	0.1278±0.0278^#
?	CP组	0.0090±0.0014	0.0278±0.0051^*	0.0406±0.0083^△★	0.0590±0.0142^☆★	0.0638±0.0146^☆#

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